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Analytica 2016
September 28-30, 2016
Volume 7, Issue 5(Suppl) J Anal Bioanal Tech 2016
ISSN: 2155-9872 JABT, an open access journal
conferenceseries .com
September 28-30, 2016 Orlando, USA
7th International Conference and Exhibition on
Analytical & Bioanalytical Techniques
Tomasz Bladek et al., J Anal Bioanal Tech 2016, 7:5(Suppl)
Multi-class method for the determination of antibacterials in honey by liquid chromatography-
tandem mass spectrometry
Tomasz Bladek, Anna Gajda, Ewelina Nowacka-Kozak and Andrzej Posyniak
National Veterinary Research Institute, Poland
Aliquid chromatography coupled with tandem mass spectrometry (LC-MS/MS) method for determination and
quantification of sulfonamides, tetracyclines, macrolides, fluoroquinolones, streptomycin, lincomycin and trimethoprim
resideues in honey has been developed. Prior to instrumental analysis, sample preparation technique involved extraction
with ammonium acetate buffer followed by solid-phase extraction cleanup using polymeric reversed phase sorbent. The
chromatographic separation was performed on C18 column with gradient elution mode. Mass spectral acquisitions were
performed under selective multiple reaction monitoring mode by triple quadrupole mass spectrometry detector. The method
has been characterized by providing the parameters of precision, accuracy, specificity, decision limit (CCα) and detection
capability (CCβ) according to Commission Decision 2002/657/EC. The calibration curves were linear in the range of 5-100 μg/
kg. The recoveries ranged from 86-104%. The repeatability was below 11.3% and within-laboratory reproducibility was lower
than 16.1%. Calculated CCα and CCβ values ranged from 5.8-7.6 μg/kg and from 6.9-9.9 μg/kg, respectively. The developed
method fulfils the criteria for confirmatory methods.
Biography
Tomasz Bladek is a Research Assistant at the Department of Pharmacology and Toxicology, National Veterinary Research Institute in Pulawy, Poland. His major
research interests include the development of new analytical methods for determination of antibiotic residues in food matrices such as muscle, milk, kidney, liver,
eggs and honey. He has published several IF papers on antibiotic residues and has been serving as a Reviewer for analytical journals.