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Analytica 2016
September 28-30, 2016
Volume 7, Issue 5(Suppl) J Anal Bioanal Tech 2016
ISSN: 2155-9872 JABT, an open access journal
conferenceseries .com
September 28-30, 2016 Orlando, USA
7th International Conference and Exhibition on
Analytical & Bioanalytical Techniques
Tahisa Marcela Pedroso et al., J Anal Bioanal Tech 2016, 7:5(Suppl)
Analytical techniques for quantifying the Ertapenem sodium with a look at green chemistry
Tahisa Marcela Pedroso1, Herida Regina Nunes Salgado1 and Ana Claudia Dantas Medeiros2
1Sao Paulo State University, Brazil
2Paraiba State University, Brazil
Ertapenem sodium is the polar and ionizable compound. Thus, the most suitable for such drug is to develop a method
for Hydrophilic Interaction Chromatography (HILIC). On the other hand, the RP-HPLC is considered as the analysis
technique used for quantification of substances and new column are often introduced for compounds separation. Two new
analytical methods have been developed. The RP-HPLC method was performed on a Waters LC system, with an Agilent™
Zorbax Bonus-RP (4.6×150 mm, 5 μm) column and the mobile phase was Water:Ethanol 80:20 v/v. While the HILIC method
was performed on an ultra-high performance liquid chromatography Shimadzu with stationary phase PhenomenexTMHILIC
Kinetex (4.6×100 mm, 2.6 µm) column and the mobile phase was Acetonitrile:Water 88:12 v/v. In both methods, 0.1% formic
acid was added in mobile phase and was performed isocratically. The validation parameters were in accordance with ICH
specifications and demonstrated accuracy, precision, selectivity, robustness and linearity. Analytical curves was constructed and
was evaluated by ANOVA (y=23043×-38525, R2=0.9999 RP-HPLC and y=29928×-547879, R2=0.9994 HILIC). The robustness
was evaluated by making parameters small alterations simultaneous and was evaluated by the Plackett-Burman. The results
show that the method validity is maintained. The methods here described have economic advantage as well as features an eco-
friendly focus. This work was developed with environmental conscience, always looking to minimize the possible generated
organic waste, which is of utmost importance to the environment.
Biography
Tahisa Marcela Pedroso has completed her Pharmaceutical honors degree with merit from the Programa Universidade Para Todos (PROUNI) rated best pharmacy
student at the Centro Universitário Central Paulista, São Carlos, Brazil in 2009. She has completed her Master’s degree in Pharmaceutical Sciences from the
Universidade Estadual Paulista, Brazil. She is currently conducting research with the antimicrobial Ertapenem sodium for the development of PhD research at the
Faculdade de Ciências Farmacêuticas, Brazil and PhD exchange student at the University of Ghent-Ugent, Belgium.