analytica-acta-2016_posters-accepted-abstracts

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Analytica 2016

September 28-30, 2016

Volume 7, Issue 5(Suppl)

J Anal Bioanal Tech 2016

ISSN: 2155-9872 JABT, an open access journal

conferenceseries

.com

September 28-30, 2016 Orlando, USA

International Conference and Exhibition on

Analytical & Bioanalytical Techniques

J Wang et al., J Anal Bioanal Tech 2016, 7:5(Suppl)

Utility of capillary microsampling for rat pharmacokinetic studies: Comparison of tail-vein bleeds

to jugular vein cannula sampling

J Wang, W Korfmacher, S Ho, Y Luo, L Shen1, Z Wu, W Sun, G Snow

and

T O’Shea

Sanofi Genzyme, USA

erial sampling methods have been routinely used for rat pharmacokinetic (PK) studies. It is still common to take 100-250 µL

of blood at each point of time when performing a PK study in rats using serial sampling. Recently, microsampling (<50 µL)

techniques have been reported as an alternative process for collecting blood samples from rats. In this report, three proprietary

compounds and two marketed drugs, fluoxetine and glipizide, were dosed orally into rats. Whole blood (and plasma) and

capillary microsampling (CMS) samples were collected from jugular vein cannula (JVC) and tail-vein from the same rats.

For the three proprietary compounds, the blood AUC as well as the blood concentration-time profile obtained from the tail

vein was different from that obtained via JVC sampling. For fluoxetine, the blood AUC was not statistically different when

comparing tail-vein sampling to JVC sampling, while the blood concentration-time profile that was obtained from the tail vein

was different than the one obtained from JVC sampling. For both fluoxetine and glipizide, the blood concentration profiles

obtained from CMS were equivalent to the blood concentration profiles obtained from the standard whole blood sampling,

regardless of the sampling site. Thus, it is recommended that a consistent blood sampling method should be used for serial

micro-sampling in discovery rat PK when testing new chemical entities. If the rat tail-vein sampling method is selected for PK

screening, a bridging study on the lead compound is recommended to confirm that PK from JVC sampling is comparable to

the tail vein sampling.