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Analytica 2016
September 28-30, 2016
Volume 7, Issue 5(Suppl) J Anal Bioanal Tech 2016
ISSN: 2155-9872 JABT, an open access journal
conferenceseries .com
September 28-30, 2016 Orlando, USA
7th International Conference and Exhibition on
Analytical & Bioanalytical Techniques
Young-Su Jeong et al., J Anal Bioanal Tech 2016, 7:5(Suppl)
Rapid detection of aerosolized Bacillus spore particles by direct in situ analysis of MALDI-TOF
mass spectrometry
Young-Su Jeong, Eugene Chong and Jong Min Lee
Agency for Defense Development, South Korea
Accurate and rapid analytical methods are essential for the detection and identification of biological warfare agents (BWA)
as well as pathogens. Although various studies have investigated the uses of a matrix-assisted laser desorption/ionization
time-of-flight (MALDI-TOF) mass spectrometry (MS) for bacterial classification, only a few studies have examined the
applicability of method for the identification of BWAs. This study aimed to generate, collect and analyze Bacillus spore aerosol
particles of 2-10 µm, the optimal size of a BWA. In this process, we developed an apparatus to directly deposit B. globigii
aerosols on MALDI target plate wells for rapid MALDI-TOF analysis. Bacillus spore aerosol particles of 2-10 µm were rapidly
analyzed using direct in situ MALDI-TOF MS without any pretreatment processes. The mass spectra of aerosolized Bacillus
spore particles were successfully detected. For real-time detection and identification, a mass spectral database of Bacillus spores
was constructed, and an algorithm was developed and applied. Bacillus spore particles were rapidly detected and identified by
MS, which can be used for the detection and inspection of BWAs such as B. anthracis spore in the battle field.
Biography
Young-Su Jeong has completed his PhD from Korea Advanced Institute for Science and Technology (KAIST) in 2012. He is a Senior Researcher of Agency for
Defense Development (ADD), ROK. He has published more than 18 papers in reputed journals.