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Volume 9, Issue 9 (Suppl) J Cancer Sci Ther, an open access journal
ISSN: 1948-5956 World Cancer 2017
October 19-21, 2017
25th WORLD CANCER CONFERENCE
October 19-21, 2017 | Rome, Italy
The effect of sex in the storage capacity of red blood cell concentrates in CPD/SAGM
Vasileios L Tzounakas1, Ariadni-Paraskevi Liakopoulou1, Violetta Anastasopoulou1, Hara T Georgatzakou1, Anastasios G Kriebardis2, Serena Valsami3,
Issidora S Papassideri1, Marianna Politou3 and Marianna H Antonelou1
1National & Kapodistrian University of Athens (NKUA), Greece
2Technological and Educational Institute of Athens
3Hematology Laboratory - Blood Bank, “Aretaieion” Hospital, NKUA, Greece
Statement of the Problem:Red blood cells (RBCs) are themost frequently transfused blood labile product.The “Donorvariation
effect”, which refers to donor-to-donor differences observed in both blood storage quality and 24h recovery, is probably a key
factor in the efficiency of transfusion therapy. Donor variation effect may be associated with genetically determined features of
RBCs and plasma. The aim of this study was to examine whether the donor’s sex may independently affect the storage capacity
of donated RBCs.
Methodology & Theoretical Orientation: For this purpose, 14 leukoreduced units of RBC concentrates in CPD/SAGM (7
male–7 female) were stored for 42 days at 4-6oC. Several parameters of storage quality (including hemolysis, redox status etc)
were examined before and throughout the storage period. SPSS was used for statistical analysis of the results.
Findings: In-bag hemolysis, as well as osmotic and mechanical hemolysis, and intracellular calcium indexes were equally low
in both groups during the whole period of storage. On the contrary, redox status markers such as total and uric acid-dependent
antioxidant capacity of the supernatant were significantly higher in male donors’ units (p<0.01). In the same group of donors,
intracellular ROS accumulation was higher during the first two weeks of storage (p<0.05), while exogenously stimulated ROS
production was higher after the middle of the storage period (797±220 vs 504±48 RFU, p<0.05).
Conclusion& Significance: Donor’s sex does not seem to affect the hemolytic parameters of the leukoreduced RBC units under
storage in CPD/SAGM. Male sex is rather associated with better extracellular antioxidant activity, but worse intracellular redox
status and increased susceptibility to exogenous oxidative stimuli. Sex may represent a genetic variant that affects some aspects
of the RBC storage lesion. This study was supported by “IKY FELLOWSHIPS OF EXCELLENCE FOR POSTGRADUATE
STUDIES IN GREECE – SIEMENS PROGRAM” to Vasileios Tzounakas.
Biography
Vasileios L. Tzounakas is a Post-doctoral researcher at the Department of Biology (Section of Cell Biology & Biophysics) of the National and Kapodistrian
University of Athens (NKUA). He has obtained Ph.D. in Cell Biology. He has served as reviewer in international journals while his main research interests include
blood transfusion biology (mainly, red blood cell storage lesion in blood products used for transfusion), erythrocyte biology in health and disease and the study of
extracellular vesicles. He has expertise in evaluating the key parameters that affect storage lesion and post-transfusion performance of red blood cells and in the
management of blood supplies in a way that will lead to the individualization of transfusion therapy. In this context, he has focused on the elucidation of storage
lesion’s features that may serve as a donor’s signature, namely ‘the donor variation effect’.
Vasileios L Tzounakas et al., J Cancer Sci Ther 2017, 9:9(Suppl)
DOI: 10.4172/1948-5956-C1-112