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conferenceseries
.com
Volume 9, Issue 9 (Suppl)
J Cancer Sci Ther, an open access journal
ISSN: 1948-5956
World Cancer 2017
October 19-21, 2017
25
th
WORLD CANCER CONFERENCE
October 19-21, 2017 | Rome, Italy
Regulation of TP63α/sonic hedgehog axis in n-dimethylnitrosamine-induced liver cancer stem cells
Hongyu Han
1
, Xiaoqian Wang
2
, Xiaoting Li
2
and
Caiyun Zhong
2
1
Sun Yat-sen University Cancer Center, China
2
Nanjing Medical University, China
N
itroso-compounds are critical dietary risk factor of liver cancer. Cancer stem cells (CSCs) play a significant role in the
formation and development of cancer. To date, the action of nitroso-compounds on the induction of liver CSCs as well
as the underlying mechanisms has not been defined. In the present study, we revealed that chronic N-nitroso dimethylamine
(NDMA) exposure induced malignant transformation of human normal liver cells. We further showed that NDMA-induced
malignant transformed liver cells exhibited CSCs properties, as evidenced by increased sphere formation capacity in serum-
free-medium culture, dramatically elevated expression of liver CSCs markers and increased number of CD133+ cells, along with
upregulation of TAp63α, downregulation of ΔNp63α, and activation of Sonic Hedgehog pathway. Moreover, we illustrated that
suppression of Sonic Hedgehog activity inhibited NDMA-induced liver CSCs properties; over expression of TAp63α activated
Sonic Hedgehog pathway and promoted liver CSCs activity, whereas down expression of TAp63α inhibited Sonic Hedgehog
and suppressed liver CSCs; an opposite action of ΔNp63α on Sonic Hedgehog pathway and liver CSCs was demonstrated.
Taken together, our data suggested for the first time the vital role of TP63α/Sonic Hedgehog axis in regulating NDMA-induced
liver CSCs, and thus could provide new insights into the molecular mechanisms of liver carcinogenesis as well as its target
intervention.
Biography
Hongyu Han has completed her PhD from Sun Yat-sen University Cancer Center in 2010. She was a research scientist in University of Pennsylvania and Fox
Chase Cancer Center during 2014-2016. Currently, she is a Scientist in the Department of Nutrition in Sun Yat-sen University Cancer Center. Her research interests
focus on the effects and mechanisms of dietary factors and cancer development. She has published more than 10 papers in reputed journals.
hanhy@sysucc.org.cnHongyu Han et al., J Cancer Sci Ther 2017, 9:9(Suppl)
DOI: 10.4172/1948-5956-C1-112