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conferenceseries
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Volume 9, Issue 9 (Suppl)
J Cancer Sci Ther, an open access journal
ISSN: 1948-5956
World Cancer 2017
October 19-21, 2017
25
th
WORLD CANCER CONFERENCE
October 19-21, 2017 | Rome, Italy
c-Met overexpression of fibroblasts increases angiogenic signal in breast cancer
Seong Gyeong Mun
Yonsei University College of Medicine, South Korea
W
e found that c-Met overexpression was induced in normal breast fibroblast (NBF) by the conditioned medium (CM)
of cancer cells regardless of subtypes, which made us to hypothesize that c-Met overexpression is a property of cancer-
associated fibroblast (CAF). Therefore, we investigated whether c-Met overexpressing NBFs contribute to tumor progression.
To this end, extracellular matrix (ECM) gene expression alteration was analyzed from the NBFs transfected with c-Met
overexpression plasmid using cDNA microarray since CAF constructs tumor microenvironment by ECM remodeling. In our
microarray data, matrix metalloproteinase 1 (MMP1) was most up-regulated in c-Met overexpressing NBF (approximately
10-fold) compared to the control NBF. According to previous studies, MMP1 induces VEGFR2 (Vascular endothelial growth
factor receptor 2) expression in endothelial cells (ECs). So, it was assumed that c-Met overexpressing NBF contributes to
breast cancer angiogenesis. In our study, the CM of c-Met overexpressing NBF induced a better tube formation of endothelial
cells (Hy926) than that of control. On the other hand, tube formation by c-Met overexpressing NBF CM was reduced in the
presence of c-Met inhibitor. Similar results were also observed in the co-culture of NBF and breast cancer cell lines (luminal
A, B, Her2, and TNBC). The expression of c-Met and MMP1 was increased by the co-culture, whereas was decreased in the
presence of c-Met inhibitor. Tube formation of EC was increased by the CM of the co-culture, whereas decreased by c-Met
inhibitor-treated co-culture CM. Based on our results, c-Met inhibitor may be able to suppress angiogenesis in breast cancer
by decreasing c-Met-induced MMP1 expression of CAFs.
Biography
Seong Gyeong Mun has completed her Bachelor’s degree in Molecular Biology from Dankook University. She is currently pursuing Master’s degree in Yonsei
University School of Medicine. She had studied about breast tumor microenvironment in her graduate school days.
msg972@yuhs.acSeong Gyeong Mun, J Cancer Sci Ther 2017, 9:9(Suppl)
DOI: 10.4172/1948-5956-C1-112