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Volume 9, Issue 9 (Suppl)

J Cancer Sci Ther, an open access journal

ISSN: 1948-5956

World Cancer 2017

October 19-21, 2017

WORLD CANCER CONFERENCE

October 19-21, 2017 | Rome, Italy

Identify specific gene associated with carcinogenesis by

Clonorchis sinensis

and N-nitroso-

dimethylamine on host cell using transcriptome sequencing

Eun-Min Kim

and

Tai-Soon Yong

Yonsei University, South Korea

lonorchissinensis

,themostprevalentparasiteinKorea,hasbeenreclassifiedasGroupIbio-carcinogenforcholangiocarcinoma

(CCA) in humans by IARC in 2009,

C. sinensis

associated cholangiocarcinoma (CCA) is still unknown. The aim of this

study was to identify distinct gene expression associated with carcinogenesis of C. sinensis. In human cholangiocyte line, H69

cells were continuously exposed to N-nitroso dimethylamine (NDMA) and excretory-secretory product of

C. sinensis

(ESP)

over one year. H69 cells that were continuously exposed to ESP of

C. sinensis

and NDMA showed cancer-like characteristics

including cell proliferation was more than 5.7 times and the proportion of cells in the G2/M phase increased up to 42%

compare to non-treated H69 cells. Moreover, the expression of the cell cycle protein E2F1 and the cell proliferation related

proteins, ki67, and cytokeratin 19 were more than 30-fold increased when NDMA and ESP were added together. Based on

these results, whole-transcriptome sequencing was performed to compare the genome-wide gene expression patterns of H69

stimulation with NDMA and/or

C. sinensis

ESP with non-treated H69. A total of 1301 differentially expressed genes (DEGs)

were identified, 521 of which were up-regulated and 780 were down-regulated. Gene ontology and Kyoto Encyclopedia of

Genes and Genomes enrichments revealed that numerous DEGs belong to cancer-relevant genes, involved in cell cycles, cell

proliferation, and cell adherent-relevant pathways. Among them, we focused on the P53 K-ras signaling genes and found that

two genes increased and eight genes decreased from a number of their genes. This result was also confirmed by real-time PCR.

In conclusion, these data suggest that the P53 and K-ras signal plays a key role in regulation of cell proliferation, which may

cause cholangiocarcinoma under stimulation by ESP of

C. sinensis

and NDMA.

Biography

Eun-Min Kim has completed his PhD from Seoul National University College of Medicine and had worked as a Research Professor in Busan National University

College of Medicine. Currently, he is studying the mechanism of cholangiocarcinoma at Yonsei University in South Korea.

eunmin76@gmail.com

Eun-Min Kim et al., J Cancer Sci Ther 2017, 9:9(Suppl)

DOI: 10.4172/1948-5956-C1-112