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Volume 9, Issue 9 (Suppl) J Cancer Sci Ther, an open access journal
ISSN: 1948-5956 World Cancer 2017
October 19-21, 2017
25th WORLD CANCER CONFERENCE
October 19-21, 2017 | Rome, Italy
Potential transcription factor involves NFE2L1 in hepatoma cell invasiveness through ROS induction
Eun-Beom Lee, Gyeong-Hyeon Kim, Young-Kyoung Lee and Gyesoon Yoon
Ajou University School of Medicine, South Korea
Mitochondrial dysfunction is an important metabolic feature in human cancer. However, underlying mechanisms how
mitochondrial dysfunction affects tumorigenesis remain unclear. To address the role of transcriptomic regulation
by mitochondrial defects in liver cancer cells, we performed gene expression profiling for three different cell models of
mitochondrial defects: cells with chemical respiratory inhibition, cells withmitochondrial DNA depletion, and liver cancer cells
harboring mitochondrial defects. By comparing gene expression in the three models, we identified 10 common mitochondrial
defect (CMD)–related genes that may be responsible for retrograde signaling from cancer cell mitochondria to the intracellular
transcriptome. Among the CMD genes, we found that NFE2L1 is a key regulator to regulate hepatoma invasiveness. This study
aims to elucidate how mitochondrial defect regulates NFE2L1 transcription. Interestingly, SNU354 and SNU423 cells showed
high intracellular reactive oxygen species (ROS) levels. Exogenous treatment of H2O2 increased intracellular ROS and NFE2L1
expression in SNU387 cell harboring active mitochondria. We further selected 11 transcription factors (TFs) that could bind
to promoter region of NFE2L1 by using TRANSFAC program. By monitoring NFE2L1 mRNA levels after knocking-down of
the TFs, 4 TFs (USF2, STAT3, JUN and SREBP1) were identified to regulate NFE2L1 transcription. Further detailed molecular
mechanisms of how mitochondrial ROS regulates NFE2L1 transcription are currently under investigation.
Biography
Eun-Beom Lee is pursuing Master's Degree fromAjou University School of Medicine. Her major Research Interest is in mitonuclear communication in tumorigenesis
and retrograde signaling in hepatoma cells.
Eun-Beom Lee et al., J Cancer Sci Ther 2017, 9:9(Suppl)
DOI: 10.4172/1948-5956-C1-112