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Volume10, Issue 12 (Suppl)
J Proteomics Bioinform, an open access journal
ISSN: 0974-276X
Page 65
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World Biomarkers & Pharma Biotech 2017
December 07-09, 2017
December 07-09, 2017 | Madrid, Spain
&
20
th
International Conference on
PHARMACEUTICAL BIOTECHNOLOGY
9
th
WORLD BIOMARKERS CONGRESS
JOINT EVENT ON
Biosynthesis of levan by using the strain
Zymomonas mobilis
ATCC 10988 in static and shaking
fermentation
Angela Casarica, Ana Despina Ionescu, Corina Bubueanu, Delia Maria Jitea
and
Maria Petrescu
National Chemical-Pharmaceutical for Research and Development Institute, Romania
L
evans are fructose polymers synthesized by a broad range of microorganisms and a limited number of plant species as non-
structural storage carbohydrates and they have potential applications in the pharmaceutical, food, and cosmetic industries.
The present study shows a comparative analyses of polysaccharide type Levan biosynthesis in static and shaking fermentation
by using
Z. mobilis
ATCC 10988 strain. All fermentation processes were carried out in Erlenmeyer flasks presenting a capacity
of 500 ml and working volume of 100 ml culture medium, in both fermentation types: static and on the rotary shaker, with
stirring of 220 rpm, with the temperature maintenance at 32°C for 72 hours. In figure 1, we can find the results obtained by
static fermentation for different initial concentrations of sucrose. The best development of the microbial culture in terms of
microbial biomass was seen for the initial concentration of 15% sucrose, due probably to the favorable ratio between the source
of C and N. The largest amount of polysaccharide was obtained from the experiment with 40% initial sucrose (8.9 g%), but
this value is also similar to the experiment with 25% initial sucrose (8.47 g%). The Figure No. 2 shows the results obtained by
the stirred fermentation with the strain
Z. mobilis
ATCC 10988 for different initial concentrations of sucrose. Concerning the
content of polysaccharide, if the initial concentration of sucrose in the biosynthesis medium was greater than 20%, the amount
of polysaccharide was about 5 g%, without any increase of production in the case of higher concentrations. By comparing
the results shown in figures 1 and 2, it can be noticed that the strain
Z. mobilis
ATCC 10988 performs better concerning the
polysaccharide biosynthesis in the frame of a static fermentation, which is not a surprise considering that these bacteria are an
optional aerobic one.
Recent Publications:
1. ParkH-E(2003)Enzymatic synthesisof fructosyl oligosaccharidesby levansucrase from
Microbacteriumlaevaniformans
ATCC 15953. Enzyme Microb. Tech. 32: 820-827.
2. Rairakhwada D (2007) Dietary microbial levan enhances cellular non-specific immunity and survival of common
carp (
Cyprinus carpio
) juveniles. Fish Shellfish Immun. 22: 477-486.
3. Gupta S (2008) Microbial levan in the diet of
Labeo rohita
Hamilton juveniles: Effect on nonspecific immunity and
histopathological changes after challenge with Aeromonas
hydrophila
. J. Fish Dis. 3: 649-657.
4. Jathore N R (2012) Microbial levan from
Pseudomonas fluorescens
: Characterization and medium optimization for
enhanced production. Food Science and Biotechnology. doi:10.1007/s10068-012-0136-8.
Biography
Angela Casarica has her expertise as a Project Responsible of different Romanian Research Projects related to Bacterial Cellulose for pharmaceutical and industrial
purposes, studies to obtain an eyewash technology based truffle extract, fungal chitosan: isolation and biological characterization, curdlan-type polysaccharide
obtained using a strain of
Agrobacterium rhizogenes
, product of natural origin for ophthalmic treatment and obtaining procedure. This work and experience in
biotechnology was attained by approximately 10 biotechnological processes, seven patents and over 50 scientific papers published in specialized journals. She is
a PhD in the field of Horticulture and she has obtained more medals at the International innovation fairs from Brussels and Geneva during the period 2008–2014.
She is working as a Senior Scientific Researcher at the National Chemical-Pharmaceutical for Research and Development Institute, Bucharest, Romania.
angelacasarica@yahoo.comAngela Casarica et al., J Proteomics Bioinform 2017, 10:12(Suppl)
DOI: 10.4172/0974-276X-C1-110