61 / 61
Volume10, Issue 12 (Suppl)
J Proteomics Bioinform, an open access journal
ISSN: 0974-276X
Page 118
conferenceseries
.com
World Biomarkers & Pharma Biotech 2017
December 07-09, 2017
December 07-09, 2017 | Madrid, Spain
&
20
th
International Conference on
PHARMACEUTICAL BIOTECHNOLOGY
9
th
WORLD BIOMARKERS CONGRESS
JOINT EVENT ON
J Proteomics Bioinform 2017, 10:12(Suppl)
DOI: 10.4172/0974-276X-C1-110
Urinary protein changes in subcutaneous walker-256 tumor-bearing rats even before tumor mass palpable
Youhe Gao
Beijing Normal University, China
E
arly diagnosis of cancer can significantly improve survival rates for cancer patients. Cancer biomarkers are measurable changes
associated with the cancer and without homeostatic control; urine reflects early changes in the body with a prospect in cancer
early diagnosis. In this study, the Walker 256 tumor rat model was established by subcutaneous injection of Walker 256 tumor cells.
To identify urinary proteome changes during the entire development of cancer, urine samples of Walker 256 tumor bearing rats were
collected at five time points corresponding to before cancer cell implant, before tumor mass palpable, tumor mass appearance, tumor
rapid growth and cachexia respectively. The urinary protein patterns on SDS-PAGE change significantly as tumors progress and
urinary proteome was identified using a Fusion-Lumos mass spectrometry by label-free quantitation. Interesting, several differential
urine proteins before tumor mass even palpable could be identified with a fold change >2 and p value <0.05, and these early changes
in urine could be also identified at tumor mass appearance, tumor rapid growth and cachexia. Twenty-four differential proteins were
annotated before as biomarkers of cancer diseases and nine proteins as biomarkers of breast cancer. Additionally, it was found that
those differential proteins were involved in several pathways related to cancer, including IL-6 and IL-12 signaling, production of nitric
oxide, ROS and apoptosis. Finally, 30 dynamically changed urinary proteins were selected as more reliable cancer biomarkers, and
they were validated by targeted proteomics. Our study suggested that urine is a sensitive biomarker source for early detection of cancer
and systemic changes reflected in urine proteome during cancer progression can improve the understanding of pathophysiological
changes of cancer.
youhegao@163.com