world-biomarkers-pharma-biotech-2017-posters

4 / 61

Notes:
Volume10, Issue 12 (Suppl) J Proteomics Bioinform, an open access journal
ISSN: 0974-276X
Page 60
conferenceseries.com
World Biomarkers & Pharma Biotech 2017
December 07-09, 2017
December 07-09, 2017 | Madrid, Spain
&
20th International Conference on
PHARMACEUTICAL BIOTECHNOLOGY
9th WORLD BIOMARKERS CONGRESS
JOINT EVENT ON
Antioxidative and anticancer activities of Julbernardia globiflora extract
Hyun Ju Kwon, You Na Oh, Soojung Jin, and Byung Woo Kim
Dong-Eui University, South Korea
Julbernardia globiflora, a tropical African tree widespread in Miombo woodland, has been used in folk medicine for the
treatment of depression and stomach problems. However, the bioactivities of J. globiflora have not yet been fully determined.
The objective of this study was to evaluate the antioxidative and anticancer effects of methanol extract of J. globiflora (MEJG)
and the molecular mechanism of its anticancer activity in human colon carcinoma HT29 cells. MEJG exhibited significant
antioxidative activity and cell growth inhibitory effect on HT29 cells in a dose-dependent manner. MEJG induced apoptosis
of HT29 cells with the increase of apoptotic cells and apoptotic bodies using Annexin V staining and 4,6-diamidino-2-
phenylindole (DAPI) staining, respectively. The MEJG-induced apoptosis was associated with the increase of Fas, a death
receptor, and Bax, a pro-apoptotic protein, and the decrease of Bcl-2, an anti-apoptotic protein, resulting in the release of
cytochrome c from the mitochondria into the cytosol and activation of caspase-3, -8 and -9. The apoptotic effects of MEJG
were confirmed by cleavage of poly (ADP-ribose) polymerase (PARP). Collectively, these results suggest that MEJG may exert
the anticancer effect in HT29 cells by inducing apoptosis via both the intrinsic and extrinsic pathways.
Recent Publications:
1. Adam J M and Cory S (2007) The Bcl-2 apoptotic switch in cancer development and therapy. Oncogene. 26: 1324-
1337.
2. Fulda S and Debatin KM (2006) Extrinsic versus intrinsic apoptosis pathways in anticancer chemotherapy. Oncogene.
25: 4798-4811.
3. Galluzzi L, Lopez-Soto A, Kumar S and Kroemer G (2016) Caspases connect cell-death signaling to organismal
homeostasis. Immunity. 44: 221-231.
4. Li P, Nijhawan D and Wang X (2004) Mitochondrial activation of apoptosis. Cell. 116: 57-59.
5. Riedl S J and Shi Y (2004) Molecular mechanisms of caspase regulation during apoptosis. Nat. Rev. Mol. Cell Biol. 5:
897-907.
Biography
Hyun Ju Kwon has completed her PhD in Bioengineering from Osaka University, Osaka, Japan. She is presently working as a Professor at Division of Applied
Bioengineering, Biopharmaceutical Engineering Major, Dong-Eui University and as an Assistant Director of Blue-Bio Industry Regional Innovation Center, Dong-Eui
University, Busan, Korea. Her research field is Natural Products and Oncology.
Hyun Ju Kwon et al., J Proteomics Bioinform 2017, 10:12(Suppl)
DOI: 10.4172/0974-276X-C1-110