world-biomarkers-pharma-biotech-2017-posters

Volume10, Issue 12 (Suppl)

J Proteomics Bioinform, an open access journal

ISSN: 0974-276X

Page 71

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World Biomarkers & Pharma Biotech 2017

December 07-09, 2017

December 07-09, 2017 | Madrid, Spain

International Conference on

PHARMACEUTICAL BIOTECHNOLOGY

WORLD BIOMARKERS CONGRESS

JOINT EVENT ON

Comparative study on the growth and consumption curves of

Zymomonas mobilis

NCIB 1163 and

mobilis

ATCC 10988, levan producer

Georgiana Gabriela Iordache, Angela Casarica

and

Ana Despina Ionescu

National Chemical-Pharmaceutical for Research and Development Institute, Romania

evans are fructose polymers synthesized by a broad range of microorganisms and a limited number of plant species as non-

structural storage carbohydrates and they have potential applications in the pharmaceutical, food, and cosmetic industries

(1-5). This study presents a comparative analysis of the growth and consumption curves of

Z. mobilis

NCIB 1163 and

Z. mobilis

ATCC 10988, levan producing microorganisms. The growth and consumption curves were performed in a liquid medium

with a concentration of 5% sucrose and 5% glucose. Thus, the bacterial cells in the exponential growth phase were centrifuged

at 12.000 g and the pellet was washed twice with a sterile 0.9% NaCl solution. Finally, the cells were resuspended in 1 ml of

physiological saline and they were used as inoculum in 5% liquid medium, which was monitored concerning the fermentable

carbohydrate consumption and the growth. The consumption curves were performed under anaerobic conditions (the culture

medium was coated with a layer of sterile paraffin oil) by periodical weighing of the medium seeded with different bacterial

strains of

Z. mobilis

and by the graphical representation of weight loss (due to release of carbon dioxide). Exponentially

growing cells were used as the inoculum, made at approximately 107 cells/ml. Cell growth was assayed turbidimetrically at

a wavelength of 600 nm. The consumption curves of

Z. mobilis

NCIB 11163 and

Z. mobilis

ATCC 10988 bacterial strains on

complete sucrose substrate medium, 5% under anaerobic conditions (Figure 1) revealed that strains NCIB 11163 and ATCC

10988 exhibit a similarly kinetics of consumption's substrate. The consumption curves of

Z. mobilis

NCIB 11163 and

Z. mobilis

ATCC 10988 bacterial strains on complete glucose substrate medium, 5% under anaerobic conditions (Figure 2) show that

glycolysis is more intense than hydrolysis of sucrose. From the curves profile it is observed that the strain ATCC 10988 shows

a curve having a more pronounced linear decrease.

Recent Publications:

1. Park H-E, (2003) Enzymatic synthesis of fructosyl oligosaccharides by levansucrase from Microbacterium

laevaniformans ATCC 15953. Enzyme Microb. Tech. 32: 820-827.

2. Rairakhwada D, (2007) Dietary microbial levan enhances cellular non-specific immunity and survival of common

carp (Cyprinus carpio) juveniles. Fish Shellfish Immun. 22: 477-486.

3. Gupta S, (2008) Microbial levan in the diet of Labeo rohita Hamilton juveniles: Effect on nonspecific immunity and

histopathological changes after challenge with Aeromonas hydrophila. J. Fish Dis. 3: 649-657.

4. Kang SA, (2009) Levan: Applications and perspectives. pp. 145-161. In: Microbial Production of Biopolymers and

Polymer Precursors. Rehm BHA (ed). Caister Academic Press, Norfolk, UK.

5. Jathore, N. R., (2012) Microbial levan from Pseudomonas fluorescens: Characterization and medium optimization for

enhanced production. Food Science and Biotechnology, 21(4), 1045–1053. doi:10.1007/s10068-012-0136-8.

Biography

Georgiana Gabriela Iordache has her expertise as a Project Responsible of different Romanian Research Projects related to Heparin Sodium for pharmaceutical

and industrial purposes. This work and experience in biotechnology was attained by approximately 10 biotechnological processes, 2 patents and over 3 scientific

papers published in specialized journals. She is a PhD student in the field of Biotechnology. She is working as a junior researcher at the National Chemical-

Pharmaceutical for Research and Development Institute, Bucharest, Romania.

iordachegeorgiana20@yahoo.com

Georgiana Gabriela Iordache et al., J Proteomics Bioinform 2017, 10:12(Suppl)

DOI: 10.4172/0974-276X-C1-110