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Volume10, Issue 12 (Suppl) J Proteomics Bioinform, an open access journal
ISSN: 0974-276X
Page 62
conferenceseries.com
World Biomarkers & Pharma Biotech 2017
December 07-09, 2017
December 07-09, 2017 | Madrid, Spain
&
20th International Conference on
PHARMACEUTICAL BIOTECHNOLOGY
9th WORLD BIOMARKERS CONGRESS
JOINT EVENT ON
Surface charge engineering of nitric oxide-releasing polymeric nanoparticles: Adhesion and anti-
biofilm efficacy against wound infection associated MRSA biofilm in db/db mice
Nurhasni Hasan
Pusan National University, South Korea
Biofilm-associated wound infections have been considered a life-threatening infection that affects millions of people each
year and are among the major cause of infectious disease-related mortality and morbidity worldwide. Bacterial biofilms
protect bacteria from host immune responses and promote strong resistance to antibiotic treatment which leads to impaired
wound healing, hospitalization and amputation particularly in chronic wound such as diabetic foot ulcer. Recently, nitric oxide
(NO) has emerged as novel agent in biofilm dispersal and accelerates wound healing. In this study, we investigated the potency
of positively charge NO-releasing PLGA/PEI nanoparticles (NO/PPNPs) for adhesion on biofilm surface that elevate biofilm
dispersal and wound healing efficacy. Poly (lactic-co-glycolic acid) (PLGA) were used to incorporate polyethyleneimine (PEI)/
NO adduct (PEI/NONOate) by an oil-in-water (O/W) emulsion evaporation method to form NO/PPNPs. Adhesion of NO/
PPNPs on bacterial biofilm and the progress of in vivo biofilm dispersal were performed in biofilm wound and characterized
by 3D confocal microscopy. In vivo biofilm was prepared by inoculating Methicillin-Resistant Staphylococcus aureus (MRSA)
suspension on the surface of wound in db/db mouse (type-2 diabetic). Photographs of the wounds were taken to observe
the gross visual wound healing. Furthermore, histological analysis was performed with H&E and Masson trichrome stain
to observe the skin morphological and collagen deposition, respectively. Positively charged of NO/PPNPs facilitated the
electrostatic binding to the negatively charged biofilm matrix, thereby increasing the biofilm dispersal by NO released from
NO/PPNPs. NO/PPNPs treatment a biofilm-challenged diabetic mouse accelerated wound healing as compared to untreated
and blank nanoparticles. In addition, histological examination revealed that wounds treated with NO/PPNPs showed increased
numbers of fibroblast-like and collagen deposition with healed skin structures close to the normal healthy epidermis. Thus, the
NO-releasing polymeric nanoparticles investigated in this study could be a promising approach for the treatment of biofilm-
challenged chronic wounds and various skin infections.
Recent Publications:
1. H Nurhasni, J Cao, M Choi, I Kim, B L Lee, Y Jung, J W Yoo (2015) Nitric oxide-releasing poly (lactic-co-glycolic
acid)-polyethylenimine nanoparticles for prolonged nitric oxide release, antibacterial efficacy, and in vivo wound
healing activity, International journal of nanomedicine. 10: 3065.
2. J S Choi, J Cao, M Naeem, J Noh, N Hasan, H K Choi, J W Yoo (2014) Size-controlled biodegradable nanoparticles:
Preparation and size-dependent cellular uptake and tumor cell growth inhibition, Colloids and Surfaces B:
Biointerfaces.122: 545-551.
3. J O Kim, J K Noh, R K Thapa, N Hasan, M Choi, J H Kim, J H Lee, S K Ku and J W Yoo (2015) Nitric oxide-releasing
chitosan film for enhanced antibacterial and in vivo wound-healing efficacy, International journal of biological
macromolecules. 79: 217-225.
4. J W Yoo, J S Lee, C H Lee (2010) Characterization of nitric oxide‐releasing microparticles for the mucosal delivery,
Journal of Biomedical Materials Research Part A. 92: 1233-1243.
5. J W Yoo, D J Irvine, D E Discher and S Mitragotri (2011) Bio-inspired, bioengineered and biomimetic drug delivery
carriers, Nature reviews. Drug discovery. 10: 521.
Nurhasni Hasan, J Proteomics Bioinform 2017, 10:12(Suppl)
DOI: 10.4172/0974-276X-C1-110