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Volume10, Issue 12 (Suppl) J Proteomics Bioinform, an open access journal
ISSN: 0974-276X
Page 64
conferenceseries.com
World Biomarkers & Pharma Biotech 2017
December 07-09, 2017
December 07-09, 2017 | Madrid, Spain
&
20th International Conference on
PHARMACEUTICAL BIOTECHNOLOGY
9th WORLD BIOMARKERS CONGRESS
JOINT EVENT ON
Layer-by-layer coated dexamethasone microcrystals for experimental inflammatory bowel disease
therapy
Murtada A Oshi, Nurhasni Hasan and Jin-Wook Yoo
Pusan National University, South Korea
Layer-by-layer (LBL) coating has gained popularity for drug delivery of therapeutic drugs. Herein, we described an approach
for enhancing the therapeutic efficiency of the locally administered dexamethasone (Dx) for the treatment of inflammatory
bowel disease (IBD). We utilized a LBL-coating technique for alternative coating of Dx microcrystals (DxMCs) with multiple
layers of polyelectrolytes composed of poly (allylamine hydrochloride), poly (sodium 4-styrene sulfonate) and Eudragit®
S100. The successful deposition of the layers onto DxMCs surfaces were confirmed through zeta potential measurement and
confocal laser scanning microscopy, while the surface morphology was investigated through scanning electron microscopy.
The drug encapsulation efficiency for LBL-DxMCs was 95% with a mean particle size of 2 µm and negative surface charge of
-45 mV. Moreover, in vitro drug release studies showed a minimum release of the drug ( 15%) at an acidic condition during
initial first 5 h followed by sustained-release at alkaline condition. For in vivo study, LBL-DxMCs were administered orally to
male ICR mice suffering from dextran sulfate sodium-induced colitis. LBL-DxMCs was found to substantially enhance anti-
inflammatory efficacy of the drug compared to uncoated DxMCs. Macroscopic, histological and biochemical (tumor necrosis
factor-α, interleukin-6 and myeloperoxidase) examinations revealed marked improvements of colitis signs in the mice treated
with LBL-DxMCs compared with those treated with uncoated DxMCs. Overall, the obtained results demonstrate that LBL-
DxMCs are an effective and safe colon-targeted delivery system for the treatment of inflammatory bowel disease.
Recent Publications:
1. Murtada A O, Abdelkarim M A and Huyam A M (2013) The effect of sodium starch glycolate concentration on
physical effectiveness of chlorpheniramine tablets. J Pharm Educ Res. 4 (1): 47-53.
2. Muhammad Naeem, Wooseong Kim, Jiafu Cao, Yunjin Jung and Jin-Wook Yoo (2014) Enzyme/pH dual sensitive
polymeric nanoparticles for targeted drug delivery to the inflamed colon. Colloids and Surfaces B: Biointerfaces. 123:
271-278.
3. Murtada A O and Abdelkarim M A (2013) Phytochemical screening and evaluation of Monechma ciliatum (black
mahlab) seed extracts as antimicrobial agents. Avicenna J Phytomed. 3 (2):126–134.
4. Murtada A O (2013) Evaluation of Physical Effectiveness of Three Brands of Diazepam Tablets Available in Sudanese
Retail Pharmacies. Int. J. of Pharm. & Research Sci. 2 (4): 642-651.
5. Muhammad Naeem, Jiafu Cao, Moonjeong Choi, Woo Seong Kim, Hyung Ryong Moon, Bok Luel Lee, Min-Soo
Kim, Yunjin Jung and Jin-Wook Yoo (2015) Enhanced therapeutic efficacy of budesonide in experimental colitis with
enzyme/pH dual-sensitive polymeric nanoparticles. Int J Nanomedicine. 10: 4565–4580.
Biography
Murtada A Oshi is pursuing his PhD at college of Pharmacy, Pusan National University, South Korea majoring in Manufacturing Pharmacy. His study in the field
of colon-specific delivery of nano and microscale drug delivery system for the treatment of inflammatory bowel disease: ulcerative colitis and Crohn's disease. He
is mainly focusing on solving out the disadvantages of traditional anti-inflammatory drugs, e.g. systemic side effects, poor targetability etc., used for the treatment
of inflammatory bowel disease. Now, he is studying the anti-inflammatory activity of different nano and microscale drug delivery systems loaded with of anti-
inflammatory drugs for the treatment of inflammatory bowel disease. He evaluates the anti-inflammatory activity of the formulations both in vitro and in in vivo.
For in vivo study of inflammation, he used experimental animal colitis using different models such as dextran sodium sulfate, dinitrobenzene sulfonic acid and
trinitrobenzene sulfonic acid.
Murtada A Oshi et al., J Proteomics Bioinform 2017, 10:12(Suppl)
DOI: 10.4172/0974-276X-C1-110