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Volume10, Issue 12 (Suppl)
J Proteomics Bioinform, an open access journal
ISSN: 0974-276X
Page 59
conferenceseries
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World Biomarkers & Pharma Biotech 2017
December 07-09, 2017
December 07-09, 2017 | Madrid, Spain
&
20
th
International Conference on
PHARMACEUTICAL BIOTECHNOLOGY
9
th
WORLD BIOMARKERS CONGRESS
JOINT EVENT ON
Anti-oxidative and anti-cancer activities of
Machaerium cuspidatum
extract
Soojung Jin, You Na Oh, Hyun Ju Kwon,
and
Byung Woo Kim
Dong-Eui University, South Korea
M
achaerium cuspidatum
, a canopy liana, is a species of genus legume in the Fabaceae family and contributes to the total
species richness in tropical rain forests. In the present study, we investigated the anti-oxidative and anti-cancer effects
of
M. cuspidatum
and the molecular mechanisms of its anti-cancer activity in human lung adenocarcinoma A549 cells and
human hepatocellular carcinoma HepG2 cells. Methanol extract of
M. cuspidatum
(MEMC) showed significant anti-oxidative
activity and the cytotoxic effect in a dose-dependent manner in several cancer cell lines. Annexin V-positive apoptotic cells and
apoptotic bodies increased by MEMC treatment. Further investigation showed that MEMC-induced apoptosis was associated
with the increase of p53 and Bax expression, and the decrease of Bcl-2 expression. In addition, MEMC treatment led to
proteolytic activation of caspase-3, -8, -9 and degradation of poly ADP ribose polymerase (PARP). Taken together, these results
suggest that MEMC may exert a beneficial anti-cancer effect by apoptosis induction via both extrinsic and intrinsic pathways
in A549 and HepG2 cells.
Recent Publications:
1. Andreas G (2003) Introduction to apoptosis. Apo Review. 2-26.
2. Fulda S (2015) Targeting apoptosis for anticancer therapy. Semin. Cancer Biol. 31:84-88.
3. Fulda S and Debatin KM (2006) Extrinsic versus intrinsic apoptosis pathways in anticancer chemotherapy. Oncogene.
25:4798-4811.
4. Halliwell B H and Gutteridge JMC (1990) Role of free radicals and catalytic metal ions in human disease: an overview.
Methods Enzymol. 186:1-85.
5. Newman D J and Cragg G M (2007) Natural products as sources of new drugs over the last 25 years. J. Nat. Prod. 70:
461-477.
Biography
Soojung Jin has completed her PhD in Immunology from Osaka University, Osaka, Japan. She is presently working as an Assistant Professor at Blue-Bio Industry
Regional Innovation Center, Dong-Eui University, Busan, Republic of Korea. Her research is focused on bioactive natural products and oncology.
microjini@deu.ac.krSoojung Jin et al., J Proteomics Bioinform 2017, 10:12(Suppl)
DOI: 10.4172/0974-276X-C1-110