49 / 61
Volume10, Issue 12 (Suppl)
J Proteomics Bioinform, an open access journal
ISSN: 0974-276X
Page 106
conferenceseries
.com
World Biomarkers & Pharma Biotech 2017
December 07-09, 2017
December 07-09, 2017 | Madrid, Spain
&
20
th
International Conference on
PHARMACEUTICAL BIOTECHNOLOGY
9
th
WORLD BIOMARKERS CONGRESS
JOINT EVENT ON
J Proteomics Bioinform 2017, 10:12(Suppl)
DOI: 10.4172/0974-276X-C1-110
DPPH and superoxide anion assay and isolation of the antioxidant compound from the methanolic extract
from the leaves of
Costus Afer
Ker-Gwal
Ibrahim H Doko
1
, Yemi H Gbadegesin
2
, Akinlolu B Fawehinmi
3
and
Victor O Fadipe
4,5
1
Raw Materials Research and Development Council, Nigeria
2
Nigerian Institute of Science Laboratory Technology, Nigeria
3
Nigerian Natural Medicine Development Agency, Nigeria
4
Federal Ministry of Science and Technology, Nigeria
5
University of Zululand, South Africa
C
ostus Afer
Ker Gwal is traditionally used in African traditional medicine to treat and manage broad spectrum of infectious
tropical disease. Apart from the medicinal uses of the plant, it has significant cultural uses such as wrapping of indigenous food
items, mat making, and feed to small ruminants. The leaves of
C. Afer
were collected from the Nigeria Natural Medicine Development
Agency, Lagos Nigeria. The leaves were dried and extracted separately with methanol and hexane. The extracts were evaluated for
antioxidant activity using DPPH and Superoxide Anion radical scavenging assays method. The methanol extract was subjected to
column chromatography eluted with dichloromethane-methanol system. The DPPH % RSA value for the methanol and hexane are
84.71 and 18.88 respectively. The methanol extracts has Superoxide Anion value of 28.15. Then harmine was isolated for the first time
from the methanol extract with the following spectroscopic data, the molecular formula C
13
H
12
ON
2
, MZ 212.1 (M+H)
+
on the basis
of its LRMS. 1H- NMR: δ 8.10 (1H, d, J=5.5 Hz), δ 7.99 (1H, d, J=8.5Hz), δ 7.80 (1H, d, J=5.5Hz), δ 7.04(1H, d, J=2.0 Hz), δ 6.87 (1H,
dd, J=8.5,2.0 Hz), δ 3.91( 3H, s), δ 2.76 ( 3H,s) and
13
C-NMR : δ 162, 144, 142, 138, 122, 114, 112, 96, 56, 29, 29, 24, 20. Literature
report indicated harmine to have broad spectrum of biological activities, this tend to validate the extensive use of
Costus Afer
in many
traditional medicine.
hdibrahim@gmail.com