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Volume10, Issue 12 (Suppl)
J Proteomics Bioinform, an open access journal
ISSN: 0974-276X
Page 101
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World Biomarkers & Pharma Biotech 2017
December 07-09, 2017
December 07-09, 2017 | Madrid, Spain
&
20
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PHARMACEUTICAL BIOTECHNOLOGY
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J Proteomics Bioinform 2017, 10:12(Suppl)
DOI: 10.4172/0974-276X-C1-110
Topical application of aqueous fraction of
Moringa oleifera
modulates the expression of inflammatory
markers and vascular endothelial growth factor (VEGF) during wound healing in diabetic rats
Abubakar Amali Muhammad
1
and
Palanisamy Arulselvan
2
1
Usmanu Danfodiyo University, Nigeria
2
Ventura Institute of Biosciences, India
4
Universiti Putra Malaysia, Malaysia
D
iabetic wound is a complication which affects significant number of people with diabetes. Its treatment is often very difficult and
the available treatments are insufficient with limited success in addition to non-affordability.
Moringa oleifera
Lam (
M. oleifera
)
from the family Moringaceae (genus Moringa) commonly called "
Drumstick tree
" is a plant traditionally employed in treatment of
many ailments and has been scientifically proven to possess hepatoprotective, anti-inflammatory, antioxidant and hypoglycemic
action. The present study was conducted to evaluate the effect of aqueous fraction of
M. oleifera
on expression level of some selected
inflammatory markers during wound healing in an animal model of diabetes. The study involved topical application of formulated
bioactive fraction of
M. oleifera
using full thickness excision wound model in Streptozotocin (STZ) and Nicotinamide (NAD) induced
diabetic rats. Thirty-six healthy adult male Wistar were divided into six groups: Two groups of normal and diabetic controls, three
groups of 0.5%, 1% and 2% w/w aqueous fraction treated and one group of positive control that received 1% w/w silver sulfadiazine
standard drug. Treatments were applied topically in cream form to the skin wounded area for 21 days. Cytokines analyses were
performed using ELISA, Western blotting and immunohistochemistry techniques. The three doses (0.5%, 1% and 2%) of bioactive
aqueous fractions were found to be significantly effective in enhancing diabetic wound healing through up regulation of Vascular
endothelial growth factor (VEGF) protein and down regulation of inflammatory mediators (TNF-α, IL1-β, IL-6, iNOS and COX-2)
in diabetic treated animals compared to untreated diabetic control (p<0.05). The downregulation of inflammatory mediators and
upregulation of VEGF by aqueous fraction facilitated overall wound healing and closure in diabetic condition. These findings suggest
that, topical administration of bioactive aqueous fraction of M. oleifera may accelerate wound healing in hyperglycemic condition.
M. oleifera, inflammatory markers, upregulation, downregulation, diabetic wound.
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