world-biomarkers-pharma-biotech-2017-posters

Volume10, Issue 12 (Suppl)

J Proteomics Bioinform, an open access journal

ISSN: 0974-276X

Page 112

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World Biomarkers & Pharma Biotech 2017

December 07-09, 2017

December 07-09, 2017 | Madrid, Spain

International Conference on

PHARMACEUTICAL BIOTECHNOLOGY

WORLD BIOMARKERS CONGRESS

JOINT EVENT ON

J Proteomics Bioinform 2017, 10:12(Suppl)

DOI: 10.4172/0974-276X-C1-110

Raphia australis

polyphenols for cancer treatment

Muyiwa Arisekola

Walter Sisulu University, South Africa

ancer, the second cause of death in theworld, has been treatedwith the use of several synthetic drugs. Some of the chemotherapeutic

drugs used in orthodox medicine include vinblastine, topotecan, irinotecan and silvestrol. All these drugs come with their

side effects like anemia, tiredness, mouth soreness, nausea, hair loss, loss of appetite, skin changes, pain, and infertility. The major

anticancer secondary metabolites are saponins, tannins and flavonoids. Due to the low toxicity, and given the fact that there has been

no literature report on polyphenols present in the genus

Raphia

, this work therefore looks into the use of

Raphia

australis

ethanol

extract in cancer chemotherapy. Extraction was done on the dried pulp of

Raphia

australis by steeping in ethanol after extracting and

concentrating, thin layer chromatography (TLC) analysis was carried out using ethanol-ethyl acetate-ammonia solvent system in the

ratio 5:3:2 respectively. Gravity column chromatography was run using this solvent system. Similar fractions were combined together

for further analysis using liquid chromatography-mass spectrometry (LC-MS). Three flavonoids and three tannins were identified

which are: afzelechin epicatechin (m/z=561.1397), catechin (m/z=289.0712), protocatechuic acid (m/z=153.0182), umbelliferone

also known as 7-hydroxycoumarin (m/z=353.0873), anthocyanidin, and quinic acid (m/z=191.0556). These phenolics all showed

anticancer activity. Due to the ubiquitous application of these polyphenols in the treatment of various forms of cancer, this work

therefore suggests the use of ethanol extract of the pulp of

Raphia australis

in the treatment of various forms of cancer. The biological

evaluation will be done using MTTS assay.