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Volume 4 Toxicology: Open Access
ISSN: 2476-2067 Toxicology Congress 2018
March 12-14, 2018
March 12-14, 2018 Singapore
14th World Congress on
Toxicology and Pharmacology
Administration of vitamin E increases BDNF and NGF in chronic stress induced rats
Ozair Hassan, Sangeetha, Kumaresan and Sankaran
Saveetha Medical College and Hospital, India
Long-term elevation of glucocorticoids hinders immune function, increasing the susceptibility to disease and neuro-
degeneration. This study evaluated the hypothesized neuroprotective effect of antioxidant and piracetam, in chronic
restraint stress induced rats. Healthy Wistar rats were divided into 4 groups (n=6) each. Group-I was kept control. Stress
group received chronic restraint stress for 6 hours per day for 21 days (Group-II). Group-III was administered vitamin E
(40 mg/kg) and group-IV was administered piracetam (336 mg/kg). Evaluation parameters were measurement of serum
brain derived neurotrophic factor (BDNF) and serum nerve growth factor (NGF). The oxidative stress markers, SOD, CAT,
glutathione peroxidase, glutathione reductase and malondialdehyde were measured. Serum nitric oxide levels were also
measured. Histological analysis of CA1 region of hippocampus was done to evaluate the structural changes of pyramidal
neurons. Spontaneous alteration behavior was analyzed using Y maze. The results revealed that vitamin E caused statistically
significant (p<0.001) increase in serum BDNF and NGF and caused statistically significant (p<0.05) increase in antioxidant
enzymes (catalase, super oxide dismutase, glutathione peroxidase, glutathione reductase), with significant (P<0.001) decrease
in malondialdehyde concentrations. Vitamin E caused increase in neuronal cell size and volume in CA1 pyramidal layer
of hippocampus and showed statistically significant (p<0.001) increase in spontaneous alteration behavior in Y maze. The
findings of study are suggestive of neuroprotection, offered by administration of vitamin E compared to piracetam against
chronic restraint stress induced rats. In conclusion naturally available dietary vitamin might serve as an adjuvant therapy in
order to avoid progression of brain damage during stress.
Biography
Ozair Hassan is currently pursuing his MBBS from Saveetha Medical College, SIMATS. His area of research is in the field of neuroscience, under the mentorship
of Dr. Sankaran P, Department of Anatomy, SIMATS in India.
Ozair Hassan et al., Toxicol Open Access 2018, Volume 4
DOI: 10.4172/2476-2067-C1-005