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Volume 10, Issue 8 (Suppl) J Proteomics Bioinform, an open access journal
ISSN: 0974-276X Structural Biology 2017
September 18-20, 2017
9th International Conference on
Structural Biology
September 18-20, 2017 Zurich, Switzerland
Hoyoung Kim, J Proteomics Bioinform 2017, 10:8(Suppl)
DOI: 10.4172/0974-276X-C1-0101
Function and structure of a chloride pump rhodopsin from marine bacteria
Hoyoung Kim
Yonsei University, Korea
Recently, light-driven sodium pump rhodopsin (NaR/KR2/NDQ rhodopsin) and chloride pump rhodopsin (ClR/NTQ
rhodopsin) from marine flavobacteria were identified by metagenomics study. One of them, light-driven sodium pump
rhodopsin (NaR) structure was determined. The other one we have solved the first crystal structure of a unique class light-
driven chloride pump (ClR) from Nonlabens marinus S1-08, at resolutions of 1.57 Å. Like structured Halorhodopsin (HR),
ClR can transfer chloride ion from extracellular to cytosol. Although both ClR and HR are same light-driven chloride pump
rhodopsin, we found some evidences that ClR and HR are different in structure and mechanism. The structures reveal two
chloride-binding sites, one around the protonated Schiff base and the other on a cytoplasmic loop. We identify a “3 omega
motif ” formed by three non-consecutive aromatic amino acids that is correlated with the B-C loop orientation. Detailed
CIR structural analyses with functional studies in E. coli reveal the chloride ion transduction pathway. Our results help to
understand the molecular mechanism and physiological role of ClR and provide a structural basis for optogenetic applications.
Biography
Hoyoung Kim has his research focus on understanding the structural and functional role of various proteins involved in cancer and immune diseases. He is
specialized in X-ray Crystallography to solve protein structures with other biophysical and biochemical techniques including Cryo_EM and SFX recently. His
ongoing research projects include various enzymes and receptors especially G-Protein Coupled Receptor (GPCR) related with cancer and immune system.
Figure1: Chloride ion conductance pathway in ClR