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Volume 7, Issue 5 (Suppl) J Biotechnol Biomater
ISSN: 2155-952X JBTBM, an open access journal Biotechnology 2017
November 13-14, 2017
November 13-14, 2017 Osaka, Japan
19th World Congress on
Biotechnology
Green synthesis of gold nanoparticles coupled with nucleic acid oxidation
Tatsuki Kunoh1, Minoru Takeda2, Syuji Matsumoto1, Ichiro Suzuki2, Mikio Takano1, Hitoshi Kunoh1 and Jun Takada1
1Okayama University, Japan
2Yokohama National University, Japan
S o-called green synthesis of safe metal nanoparticles, especially gold nanoparticles (AuNPs), has increased in importance
for medical and pharmaceutical applications. Thus, a variety of ecofriendly, energy- and cost-saving techniques have been
developed. Here, we show that RNA prepared from Leptothrix (iron-oxidizing bacteria) cells can reduce Au(III) and spherical
AuNPs eventually form when an aqueous solution of Au chloride (HAuCl4 solution) is added under ambient conditions. RNA
and DNA of other organismal origins have the same ability. Of the nucleosides and nucleobases, only guanosine and guanine
can form AuNPs. The DNA moiety, 2'-deoxyguanosine (dG), used as a reference material, forms AuNPs when mixed with
HAuCl4 solution, but 8-hydroxy-2'-deoxyguanosine (8-OHdG) does not, indicating that AuNP-formation evidently depends
on the reduction potential of the guanine moiety, not the sugar moiety. This finding is the first demonstration that spherical
AuNPs of ca. 5 nm diameter can be obtained by simply adding guanine to HAuCl4 solution at ambient temperature and no
other chemicals or physical treatments are needed.
Biography
Tatsuki Kunoh is presently an Associate Professor of Graduate School of Natural Science and Technology, Okayama University, Japan. He received his BSc
in Biotechnology, MSc and PhD degrees in yeast cell biology at Osaka University. He accumulated his experiences in Molecular Biology and Biochemistry in
Albert Einstein College of Medicine, USA and other Universities, Japan. Currently, he is a member of the Government-granted project-Toward creating innovative
applications to harness the novel functions of nano-scaled iron oxides of microbial origin in CREST supported by JST and his research focuses on the biomaterial
science.
Tatsuki Kunoh et al., J Biotechnol Biomater 2017, 7:5 (Suppl)
DOI: 10.4172/2155-952X-C1-083