9 / 24
Page 45
conferenceseries
.com
Volume 7, Issue 5 (Suppl)
J Biotechnol Biomater
ISSN: 2155-952X JBTBM, an open access journal
Biotechnology 2017
November 13-14, 2017
November 13-14, 2017 Osaka, Japan
19
th
World Congress on
Biotechnology
Effect of transdermal delivery of gelatins on the partial obesity in high fat diet induced obese rats
Sung-Min An
Pusan National University, South Korea
P
artial obesity is a constitutional disorder of fatty tissue deposit. It is not just a cosmetic problem due to the development
of the metabolic syndromes. In the present study, we developed the new method to treat partial obesity by attachment of
microneedles (MNs). In addition, the effects of gelatin on fat metabolism in adipocytes are addressed. Firstly, we applied MN to
high fat diet (HFD) induced obese rats and examined rat amount of subcutaneous adipose tissue (SAT) by histological analysis
and measured by micro-computed tomography (μCT) scanner. Also, expression of genes-associated with lipid metabolism is
analyzed in isolated adipocytes and SAT using Q-PCR and western blotting experiments. In addition, lipolysis was measured
by determining the amount of glycerol released from adipocyte. In our results, histological analysis demonstrated that the
amount of SAT stored in adipocytes was reduced by MN. For the next results, we found that administration of gelatin and MN
suppressed the expression levels of lipid metabolism-associated genes in both the isolated adipocytes and rat SAT. In addition,
glycerol release levels which is an indication of lipolysis, was also increased in isolated adipocytes. These findings suggest that
MN induces lipolysis, leading to the release of glycerol and regulates lipid metabolism and fat deposition in the AT, thereby
reduces SAT in HFD induced obese rats. Our results suggest that the alteration of lipid metabolism and fat deposition through
the application of MN may help to reduce SAT.
Biography
Sung-Min An has completed his Master’s course from Department of Biomaterials Science, Pusan National University, South Korea.
ied234@naver.comSung-Min An, J Biotechnol Biomater 2017, 7:5 (Suppl)
DOI: 10.4172/2155-952X-C1-083