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conferenceseries
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Volume 7, Issue 5 (Suppl)
J Biotechnol Biomater
ISSN: 2155-952X JBTBM, an open access journal
Biotechnology 2017
November 13-14, 2017
November 13-14, 2017 Osaka, Japan
19
th
World Congress on
Biotechnology
Pregnenolone as a new hormone therapy for female diseases targeting ERβ
Ye Young Shin
Pusan National University, South Korea
M
any steroid hormones such as estrogen (E2) bind their receptors to regulate biological processes. Pregnenolone (PG) is the
precursor from almost all of the other steroid hormones and is used for skin disorders and reproductive complications.
However, mechanism and function of PG are not well established in the uterus. In this study, we examined the effects of PG on
the activation of estrogen receptor (ER) in uterus. First, we performed computational structure prediction of PG-ER complexes
and PG showed high affinity with ERβ. To study the mechanism of PG directly, Ishikawa cells were transfected with ERE-
luciferase plasmid and isoforms of ERs. ERE-luciferase activity induced by PG was similar with E2 and showed high activity
with ERβ plasmid. Also, the expression of ERα target genes in cells treated with PG was reduced compared to E2. In immature
rat, PG negatively regulated the expression of ERβ in the uterus. These findings suggest that PG stimulates ERβ-mediated
signaling in the uterus. Activation of ERβ by PG may help to overcome ERα-related female reproductive diseases such as
endometriosis, breast cancer and ovarian cancer.
Biography
Ye Young Shin has completed her Bachelor's degree from Department of Biomaterials Science, Pusan National University, South Korea.
bubu3935@naver.comYe Young Shin, J Biotechnol Biomater 2017, 7:5 (Suppl)
DOI: 10.4172/2155-952X-C1-083