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Volume 9, Issue 9 (Suppl)
J Cancer Sci Ther, an open access journal
ISSN: 1948-5956
World Cancer 2017
October 19-21, 2017
25
th
WORLD CANCER CONFERENCE
October 19-21, 2017 | Rome, Italy
Inhibitory effect of
Lactobacillus helveticus
SBT2171 on the growth of colon carcinoma cells and the
novel action mechanism
Kazunobu Baba
Hokkaido University, Japan
L
actobacillus helveticus
SBT2171 (LH2171: provided by Megmilk Snow Brand Co., Ltd.) is a lactic acid bacterium that
inhibits excessive immune responses. We have revealed that LH2171 shows inhibitory effects on the proliferation of BJAB,
a B lymphoma cell line, through reduction of phosphorylation level of c-Jun, a critical regulator of cell proliferation. c-Jun is
phosphorylated by activation of MAPKs (Mitogen-activated protein kinases) - (JNK and ERK) signaling pathway. JNK (Jun
N-terminal kinases) and ERK are activated byMEK4/7 or MEK1/2 pathway, respectively. In this study, we investigated the effect
of LH2171 on the proliferation of MC38, a mouse colon carcinoma cell line and HT-29, a human colorectal adenocarcinoma
cell line. LH2171 inhibited the proliferation of both MC38 cells and HT-29 cells. As a result of assay for the phosphorylation
of factors regulating cell growth, LH2171 inhibited phosphorylation of JNK, but not of ERK and MEK4 in MC38 cells. In
addition, LH2171 induced the expression of JNK-inactivating phosphatase, MKP1 and MKP1 siRNA treatment could suppress
the effect of LH2171 to inhibit cell proliferation. These results indicate that the inhibitory effect of LH2171 on the proliferation
of MC38 cells depends on the induction of MKP1 to inhibit JNK activity.
Biography
Kazunobu Baba has completed her PhD from Kwansei Gakuin University. She is a Postdoctoral Fellow of Institute for Genetic Medicine in Hokkaido University.
She has been studying the function and roles of probiotics to apply the study in prevention and treatment of cancer. She has once received Paper of the Week on
Journal of Biological Chemistry.
k-baba@pop.med.hokudai.ac.jpKazunobu Baba, J Cancer Sci Ther 2017, 9:9(Suppl)
DOI: 10.4172/1948-5956-C1-112