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Journal of Biotechnology & Biomaterials | ISSN: 2155-952X | Volume: 8
3
rd
World Biotechnology Congress
December 03-04, 2018 Sao Paulo, Brazil
Feasible technical and economic solutions for EPA production by
Pythium irregulare
using vinasse,
wastewater, as main raw material
Bruna S Fernandes
1
, Bruno C Klein
2
, Joao Paulo Fernandes Vieira
3
, Reinaldo Ferreira
4
, Marcelo Zaiat
5
, Rubens Maciel Filho
1
, José GC Pradella
5
1
University of Campinas, Brazil
2
Brazilian Centre of Research in Energy and Materials, Brazil
3
Raizen, Brazil
4
Dedini S/A Indústrias de Base, Brazil
5
University of São Paulo, Brazil
V
inasse is a rich carbon source and low-cost feedstock produced in huge amounts from the process of ethanol production. In
2019, the Brazilian Ministry of Agriculture, Livestock and Food Supply estimate growth of ethanol domestic consumption
of 58.8 billion liters, more than double the amount recorded in 2008. This represents the annual production of more than 58.8
billion liters of vinasse, which is currently used as fertilizer in the sugarcane crop, due to its high concentration of minerals,
mainly potassium. However, studies indicate some disadvantages such as the generation of Greenhouse Gas emission
during vinasse distribution in the crop, as well as the possibility of contaminating the groundwater and soil. Therefore, the
development of programs for sustainable use of vinasse is a priority. One profitable process is the bioconversion of vinasse into
biocompounds such as lipids, by microorganisms. Promising high value-added lipids, for instance, polyunsaturated fatty acids
(PUFAS), with a predicted market of millions of US$, can be produced using vinasse as a carbon source, to guide an innovative
e feasible concept for sustainable production. In this context, Omega 3 Eicosapentaenoic acid (EPA), a PUFA, not synthesized
by humans but an important dietary supplement with a promising market, was produced by
Pythium irregulare
, an oleaginous
Oomycete, able to accumulate large amounts of lipids and the production was evaluated according to several technical and
economic solutions and scenarios. The results of this study showed a great alternative for EPA production applied to human
consumption, considering different microbial bioreactors configuration, alternative extraction and refinement processes.
Biography
Bruna Soares Fernandes graduated from University Federal of São Carlos (Brazil) on chemical engineering. She did Master and Doctoral degree at University
of São Paulo (Brazil) on Hydraulic and Sanitation engineering. She worked at Dedini/Paques BV as new technologies leader focused on wastewater treatment.
During the four last years, she got a position as Technical Coordinator of R&D project of Ergostech, Sapporo Brewery and Petrobras. She and her team developed
a process, in which it was patented. Since 2015, she is in a Postdoctoral position at the Brazilian Centre of Research in Energy and Materials and University of
Campinas. Her main interests are waste treatment and bio-products obtainment from biological processes.
brunasofer@hotmail.comBruna S Fernandes et al., J Biotechnol Biomater 2018, Volume: 8
DOI: 10.4172/2155-952X-C7-106