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Journal of Biotechnology & Biomaterials | ISSN: 2155-952X | Volume: 8
3
rd
World Biotechnology Congress
December 03-04, 2018 Sao Paulo, Brazil
Mannosylerythritol lipids production by
Pseudozyma aphidis
UFMG-Y3468: A process optimization
approach
Glaucia M Pastore
1
, Bruno N Paulino
and
Carlos A Rosa
2
1
University of Campinas, Brazil
2
Federal University of Minas Gerais, Brazil
Statement of the Problem:
Yeasts from Ustilaginaceae family have been highlighted in the last years as a promising source
of industrial interesting compounds, including enzymes, sugars, lipids, organic acids and glycolipids biosurfactants. Among
the biosurfactants produced by these yeasts, the mannosylerythritol lipids (MELs) have been attracted the attention of the
scientific community in recent years due to its potential applications in several fields including medical, cosmetics and food.
Considering the high costs associated with the production of biosurfactants, this work focused on the use of response surface
methodology (RSM) to optimize the carbon and nitrogen sources in the medium for the production of MELs by a
Pseudozyma
aphidis
strain isolated from Brazilian water sample.
Methodology & Theoretical Orientation:
After a screening study, one strain of yeast belonging to the
Pseudozyma
genus
showed able to produce MEL in mineral medium with glucose. A central composite design (24), 28 experiments, was used for
optimization of glucose, soybean oil, yeast extract and sodiumnitrate concentrations inmineral medium applied for production
of MELs by
P. aphidis
UFMG-Y3468. The process was carried in a controlled incubator at 30°C and 200rpm for 10 days.
Findings:
The production of crude MEL extract ranged of 5,87g/L to 27,25g/L and the mathematical model obtained with
significative parameters (soybean oil, glucose and yeast extract) showed good results after analysis of variance. In addition,
after the chemical characterization using high-resolution mass spectrometry and nuclear magnetic resonance was observed
the presence of MEL-A, MEL-B, MEL-C.
Conclusion & Significance:
The use of RSM for optimization of MEL production can be considered an important tool to
reduce of costs of medium composition applied in the biotechnological processes. This approach can be considered promising
since it allowed to find the optimum concentration levels of the substrates used for MEL production.
Biography
Glaucia M Pastore, Full Professor, Department of Food Science, Faculty of Food Engineering, Campinas University, UNICA Bachelor of Biological Science,
Catholic University of Campinas (1976) MS in Food Science, Campinas University-Unicamp (1982), Enzyme Technology Course Osaka Technical Institute Japan
(1985), PhD in Food Biochemistry, Campinas State University-Unicamp (1991), Post-doctorate: Ohio State University USA, 1993. Responsible for graduate
and undergraduate courses in Food Biochemistry and Principes of Food Biotechnology, Biotransformation of Agriculture wastes. Author of several chapters in
international and national books, more than 100 research papers in international journals.
glaupast@fea.unicamp.brGlaucia M Pastore et al., J Biotechnol Biomater 2018, Volume: 8
DOI: 10.4172/2155-952X-C7-106