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Journal of Biotechnology & Biomaterials | ISSN: 2155-952X | Volume: 8
3rd World Biotechnology Congress
December 03-04, 2018 Sao Paulo, Brazil
Attenuated Salmonella strains have showed great potential as live vectors with broad applications in
veterinary medicine
Luciana Helena Antoniassi da Silva
University of Campinas, Brazil
Salmonella belong to the Enterobacteriaceae family are enteric Gram-negative and facultatively anaerobic bacteria that
comprises pathogens of worldwide economic and health importance. The symptoms range from gastroenteritis to severe
systemic fevers they cause disease in several animals such as mammals, birds and reptiles. Attenuated Salmonella strains have
been developed as live vaccines for humans and animals to prevent disease caused by Salmonella infections. By introduction of
mutations in the genes or deletions of genes that are essential for metabolism, virulence or survival in the host organism. Live
vaccine vehicles offer a powerful approach for inducing protective immunity against pathogenic microorganisms. Genetically
engineered and attenuated agents provide a method for delivering heterologous antigens derived from other pathogens. Main
objective was: characterization the ihfA and ihfAB mutants constructed from S. enterica Typhimurium strains marked with
luminescence by introducing the luxCDABE operon using bioluminescence imaging technique, in which we assessed the
dynamics of colonization of these mutants compared to the wild-type strain, confirming the attenuation profile our mutants.
Methodology: One-day-old chicks andmicewere orally immunized through crop of 0.1mLwithSalmonella Typhimuriumwild-
type group, single mutant ihfA group and double mutant ihfAB group. The control group was kept as nomimmunized control
and was given 0.1 mL sterile PBS group. We compare the attenuation of serotype Typhimurium IHF mutants in two different
animal models. Ours results show the attenuation after oral infection in these two animal models chickens and murine. Our
findings suggest that future studies of both the chick and murine are needed to determine the role of this important microbial
community in the differential development of gastrointestinal disease caused by Salmonella and on containing systemic spread
of these organisms in different host species.
Biography
Luciana Helena Antoniassi da Silva has her PhD in Genetics and Molecular Biology. She has her expertise in molecular biology and strong experience in
microbiology with emphasis on Virology working mainly with human and animal respiratory viruses. She has knowledge in biotechnology and genetic modification
of microorganism like Salmonella.
Luciana Helena Antoniassi da Silva, J Biotechnol Biomater 2018, Volume: 8
DOI: 10.4172/2155-952X-C7-106