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Journal of Biotechnology & Biomaterials | ISSN: 2155-952X | Volume: 8
3rd World Biotechnology Congress
December 03-04, 2018 Sao Paulo, Brazil
Evaluating the feasibility of the direct L-Ascorbic acid synthesis using a one-stage process and a
single strain of Pleurotus ostreatus
Jorge L Betancurt, Juan M Parra, Diego A Montoya, Héctor J Osorio and Susana Hernández
National University of Colombia, Colombia
L-ascorbic acid (L-AA) also known as vitamin C is currently produced on a commercial scale by a complex and costly
two-stage process which involves two chemical catalysis steps and two aerobic fermentation procedures. To simplify the
industrial production of L-AA by a one-stage, single-strain process has been a major research goal for nearly three decades, but
this has not yet been accomplished. In this study, the direct L-AA synthesis using a one-stage process and a Pleurotus ostreatus
strain was evaluated on a laboratory scale. Inoculation of the strain for induction of mycelial growth was done on sucrose-
asparagine (SA) and yeast malt extract (YM) media to determine the more suitable nutrient conditions. The vegetative cultures
were grown in the dark at 25 oC for 10 days. Fungal mycelia were harvested from YM agar plates and the L-AA content was
extracted with 5% metaphosphoric acid and analyzed using high performance liquid chromatography (HPLC). Our results
show that invasive mycelial growth occurred only on YMmedium. On a fresh weight (fw) basis, the P. ostreatus mycelia contain
97.17 mg/100 g of L-AA. Our findings indicate that the direct L-AA synthesis using a one-stage process and a single strain of
P. ostreatus is feasible.
Biography
Jorge L. Betancurt is an undergraduate student at Universidad Nacional de Colombia Manizales Campus, Colombia, South America. I am expected to receive my
Chemical Engineer degree in September 2020. I joined the Bioproducts Research Group of Susana Hernandez at the same University in February 2017 to work
on the cultivation of Pleurotus mushrooms and quantification of vitamin C and provitamin A in Passiflora species cultivated in Colombia using HPLC. The purpose
of the Bioproducts Research Groups is to provide information that can be applied in biotechnology to simplify industrial processes and solve health and nutrition
problems..
Jorge L Betancurt et al., J Biotechnol Biomater 2018, Volume: 8
DOI: 10.4172/2155-952X-C7-106