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Volume 7, Issue 6 (Suppl)
J Biotechnol Biomater, an open access journal
ISSN: 2155-952X
World Biotechnology 2017
December 04-05, 2017
2
nd
World Biotechnology Congress
December 04-05, 2017 | Sao Paulo, Brazil
Bauhinia
stem extracts as a possible new treatment for breast cancer and metastasis: Inhibition of
migration, invasion and of the activity of matrix metalloproteinases
Santos K M
1
, Gomes I N F
1
, Romão W
1
, Ribeiro R I M A
1
, Silva-Oliveira R J
2
, Pinto F E
3
, Oliveira B G
3
and
Reis R M V
4
1
Federal University of São João del Rei, Brazil
2
Barretos Cancer Hospital, Brazil
3
Federal University of Espírito Santo, Brazil
4
University of Minho, Portugal
M
etastasis is the main cause of cancer-related death and requires the development of effective treatments with reduced toxicity
and effective activity. The breast cancer is the most common among women and the second most prevalent type in the entire
population. Thus the search for new sources of antitumor and anti-metastatic therapies, such as plants, is very important. In this work,
we showed the antitumor and antimetastatic activities of four fractions (ID7, IID10, IA19 and IIIA32) of the stems of
Bauhinia
species
on the murine breast cancer line 4T1. These fractions were used because they completely inhibited the MMP-2 and MMP-9 activity.
The viability assay (MTT, Trypan blue) showed that all fractions studied decreased the viability of 4T1 cells, being the ID7 fraction
the most selective. The fluorescence microscopy assay with acridine orange and propidium iodide showed that fractions increased
the apoptotic cells percent. The wound closure and trans well assays were used to evaluate the migration cell, and the trans well assay
with Matrigel was used to evaluate the invasion cell, wherein all fractions inhibited the 4T1 wound closure, IID10, IA19 fractions
inhibited trans well migration, and ID7, IID10, and IIIA32 decreased invasion cell. Furthermore, all fractions increased the 4T1
adhesion to basement membrane components and decreasing the MMP-2 activity in the 4T1cells supernatant. In the in vivo assay,
this fraction decreased the volume and weight of the tumor extracted from mice induced with 4T1 and treated with ID7, in addition
to decreasing the number of lung metastases. The ID7 ESI-MS(-) characterization suggesting the presence of fatty acids, phenolic
acids, and diterpene. Thus, it was found that the
Bauhinia
fractions tested, exhibited selective antitumor and antimetastatic activity
of breast cancer.
Biography
Santos K M is a Biomedical from José do Rosário Vellano University (2010). She has completed her Master's degree in Biotechnology from the Federal University of São
João del Rei (2013). Currently, she is a PhD student in Biochemistry and Molecular Biology by Multicentral Program of the Brazilian Society of Biochemistry and Molecular
Biology, working on the main themes: medicinal plants (Bauhinia) fractionation and identification of compounds, cancer and metastasis (metalloproteinases), Alzheimer
(acetylcholinesterase inhibition), cryopreservation and culture of tumor cells and depression (behavioral assays). She is also Professor at the Biomedicine and Physiother-
apy courses at the José do Rosário Vellano University and at the University Center of Formiga.
kamillamonteiro07@gmail.comSantos K M et al., J Biotechnol Biomater 2017, 7:6 (Suppl)
DOI: 10.4172/2155-952X-C1-085