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Volume 7, Issue 6 (Suppl)
J Biotechnol Biomater, an open access journal
ISSN: 2155-952X
World Biotechnology 2017
December 04-05, 2017
2
nd
World Biotechnology Congress
December 04-05, 2017 | Sao Paulo, Brazil
Antitumor activity of Miconia chamissois Naudin fractions in human glioma lines:
In vitro and in vivo
Ana G Silva
1
, Fernanda E Pinto
2
, Wanderson Romão
2
, Alisson R Rezende
3
, Fernanda P Cury
4
, Viviane A O Silva
4
, Renato J S Oliveira
4
, Rui M V Reis
4
and
Rosy
I M A Ribeiro
4
1
UFSJ-CCO, Brazil
2
UFES, Brazil
3
UEMG, Brazil
4
HCB–CPOM, Brazil
Introduction:
Gliomas represent nearly 70% of the central nervous system tumors. Despite the progress of chemotherapy and
radiotherapy, the median survival is around 12-17 months. Studies have shown that the use of new natural antineoplastic agents has
been highly effective and offers a wide field of research. The aim of this study is to investigate the antitumor potential of
M chamissois
Naudin chloroform partition and fractions on glioma cell lines.
Results & Discussion:
The chloroform partition and fractions exhibited dose-dependent cytotoxic effects in the majority of the
glioma cell lines. Amongst the fractions tested, McC1 and McC3 displayed the best activity, with an IC
50
mean ranging from 0.25
to 30 µg/mL and index selectivity. These fractions also showed a significant reduction in cell migration (35% for McC1 and 24% for
McC3), and invasion (24% for McC1 and 22% for McC3). Furthermore, the clonogenicity was reduced 40% for GAMG and 50% for
U251MG with McC1. Both fractions had a synergistic effect when combined with the chemotherapeutic temozolomide. The fractions
promoted a significant increase of pH2AX, cleaved PARP and cleaved caspases (3, 7 and 9) levels (p<0.05), suggesting DNA damage
and cell death by apoptosis.
In vivo
chicken chorioallantoic membrane assay, the McC1 fraction inhibited angiogenesis and tumor
perimeter. The two best fractions McC1 and McC3 were characterized by electrospray ionization mass spectrometry, both containing
six molecules.
Conclusion:
These findings contribute to new treatments for human glioblastoma, in addition to the combination with conventional
therapy potentiate its therapeutic effect.
Biography
Ana G Silva is a PhD student in the Biotechnology program of the Federal University of São João del-Rei. She has completed her Master's degree in Biotechnology Applied
to Health (2017) and Bachelor's degree in Nursing (2014) from the Federal University of São João del-Rei, Campus Centro Oeste Dona Lindu, Divinópolis/MG. She par-
ticipates in the research group Biological Activity of natural products. It currently focuses on screening new compounds from natural products
in vitro
and
in vivo
in human
glioblastoma tumor cell lines.
silvaa.gabriela@yahoo.com.brAna G Silva et al., J Biotechnol Biomater 2017, 7:6 (Suppl)
DOI: 10.4172/2155-952X-C1-085