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Volume 7, Issue 6 (Suppl)
J Biotechnol Biomater, an open access journal
ISSN: 2155-952X
World Biotechnology 2017
December 04-05, 2017
2
nd
World Biotechnology Congress
December 04-05, 2017 | Sao Paulo, Brazil
Anti-cancer drug discovery: Rational strategy to acquire anti-cancer candidate compounds
Rosy I M A Ribeiro
Federal University of São João del Rei, Brazil
Statement of the Problem:
Cancer is a set of malignancies that has in common the exacerbated and uncontrolled cellular growth,
as well as the capacity of cellular invasion to different organs of the primary site. Neoplastic transformation occurs through the
acquisition of characteristics related to proliferation, invasion, metastasis, resistance to death, genomic instability, among others. Our
research group has as main objective the search of new molecules that act inhibiting these pathways.
Methodology:
Cell viability assay, combination assay, morphological analysis, proliferation assay, wound healing assay, cell cycle
analysis, gelatin zymography, matrigel invasion assay, soft agar colony assay, acridine orange staining, detection of mitochondrial
membrane potential, comet assay, western blot,
in vitro
retina model,
in vivo
chick chorioallantoic membrane (cam) assay and in vivo
models for studying breast cancer development, and thin layer chromatography and spectrometry of masses.
Findings:
We have been able to screen samples from the production of crude to fractionated extracts that are selectively cytotoxic to
tumor cells. In addition, we determine the major pathways by which these cells die. We determined whether these treatments cause
a change in the pattern of migration and cellular invasion and the involvement of the matrix metalloproteinases 2 and 9 in these
processes. We selected several samples that will be submitted to
in vitro
cytotoxicity tests. Finally, with human cell lines, we determine
the angiogenic capacity and tumor growth by CAM. If the cell line is murine, we carry out
in vivo
tests in mice. The better samples
are directed to bioassay-guided purification that involves diverse chromatography methods. In addition, if the active substance is
unpublished, it is directed to identification by appropriate chemical techniques.
Conclusion & Significance:
By combining such approaches, we maximized the selection of molecules potentially relevant for the
discovery of anticancer molecules.
Biography
Rosy I M A Ribeiro has obtained her postgraduate degree from the University of Minas Gerais (UFMG) in Pathology. She is an Associated Professor of Cell Biology at Med-
icine Department of Federal University of São João del Rei (UFSJ), Brazil. Currently, she is the Coordinator of the Biotechnology Graduate Program of UFSJ. Her research
and publications are about the action of both synthetic and natural products on pathological processes such as wound healing and cancer.
rosy@ufsj.edu.brRosy I M A Ribeiro, J Biotechnol Biomater 2017, 7:6 (Suppl)
DOI: 10.4172/2155-952X-C1-085