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Volume 7, Issue 6 (Suppl)

J Biotechnol Biomater, an open access journal

ISSN: 2155-952X

World Biotechnology 2017

December 04-05, 2017

World Biotechnology Congress

December 04-05, 2017 | Sao Paulo, Brazil

Comparative analysis of two inducible promoters for controlled nuclear transgene expression in

Chlamydomonas reinhardtii

Paula Barjona do Nascimento Coutinho, Christine Friedl Rainer Buchholz

and

Stephanie Christine Stute

Friedrich-Alexander-Universität Erlangen-Nürnberg, Germany

enetically, well characterized microalgae like

Chlamydomonas reinhardtii

offer the potential to photosynthetically produce high

value products such as recombinant proteins for the pharmaceutical and chemical industry. Several attempts have been made to

enhance expression of foreign genes in this green alga and in principle, allow protein production at large scale. However, satisfying

and economically attractive levels of recombinant gene products have not been achieved yet. Inducible promoters represent a useful

alternative to optimize protein yield. By providing regulated gene expression, they allow the biosynthesis of gene products at most

suitable moments of cultivation, guaranteeing higher space-time yields. In this study, two inducible promoters were compared. We

demonstrate the kinetics of induction and deactivation of the iron-responsive

Fea1

promoter and the ammonium/nitrate-responsive

Nit1

promoter in the green alga

C. reinhardtii

via the fluorescent protein mCherry and detection of mRNA levels through qPCR. Our

work lays the foundation for the establishment of a cyclic process in which promoter activity is activated and deactivated alternately

by changes in the iron and ammonium concentrations in the culture media. Fluorescence microscopy picture of

C. reinhardtii

cells

expressing mCherry under the control of the

FEA1

promoter

Biography

Paula Barjona do Nascimento Coutinho has her expertise in genetic transformation of the green alga

Chlamydomonas reinhardti

and the methods developed for the detec-

tion of the fluorescent reporter protein mCherry (flowcytometry, western blot and fluorescence microscopy).

Paula Barjona do Nascimento Coutinho et al., J Biotechnol Biomater 2017, 7:6 (Suppl)

DOI: 10.4172/2155-952X-C1-085