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Volume 4 Toxicology: Open Access
ISSN: 2476-2067 Toxicology Congress 2018
March 12-14, 2018
March 12-14, 2018 Singapore
14th World Congress on
Toxicology and Pharmacology
Hyaluronic acid hydrogel as drug carrier for rheumatoid arthritis treatment
Jiyoung Seo
Ajou University, South Korea
Patients with rheumatoid arthritis-related diseases show an increasing
trend. Although many drugs for rheumatoid arthritis (RA) have been
appeared in the market, the drugs have some disadvantages; short half-
life in vivo, inconvenience of taking drugs for a long period of time and
drug toxicity in oral administration. Thus, a study of formulations that
can continuously release the drug is being required. Recently, injectable
drug carriers into the joints as a treatment for RA have been suggested
as a very effective treatment. Therefore, in this study, we have developed
the effective drug carrier via hyaluronic acid (HA), which has a high
bioavailability, for RA treatment. The cross-linking agent was introduced
into HA having a molecular weight of 1,000,000 to improve the physical
properties of the HA hydrogel and control the pore size of each HA
hydrogel for continuous drug release. We also investigated cell viability
and inflammation test by using Alamar blue assay and enzyme-linked immune-sorbent assay (ELISA) about RAW264.7 cells
and SW 982 cells. By near-infrared (NIR) fluorescence imaging, we confirmed the local release of NIR from the depot injected
into the articular joint over an extended period. The effect of HA hydrogel as a RA drug delivery depot was evaluated in vivo
experiments through extraction and staining over 1 week, 3 weeks and 6 weeks. Collectively, these results indicated that the
drug depot formed after intra-articular injection of methotrexate loaded cross-linked HA hydrogel induced long-lasting drug
release and allowed to result in enhanced RA repair.
Biography
Jiyoung Seo majored in Applied Chemistry and Biotechnology at Ajou University and obtained a Bachelor of Science (BS) in Engineering (2017). She was selected
as the Research Director of the program sponsored by the Ministry of Science and ICT and Korea Research Foundation. In the program, she studied the delivery
of rheumatoid arthritis medications. She is currently pursuing Master’s degree in the Ajou University Regenerative Medicine Laboratory.
Jiyoung Seo, Toxicol Open Access 2018, Volume 4
DOI: 10.4172/2476-2067-C1-006
Figure-1: Hydrogel loaded dual syringe. Hyaluronic acid hydrogel
(HA, each introducing crosslinking agent) loaded in dual syringe.
The HA including Methotrexate is cross-linked through the needle
of a syringe. The hydrogel becomes a better viscous drug carrier for
treatment of Rhematoid arthritis.