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Volume 4
Toxicology: Open Access
ISSN: 2476-2067
Toxicology Congress 2018
March 12-14, 2018
March 12-14, 2018 Singapore
14
th
World Congress on
Toxicology and Pharmacology
Influence of immune modulation by tumor cell-derived soluble compound
Mohammad Amjad Hossain, Adithan Aravinthan, Judith Sharmila, Bumseok Kim, Chang Won Kang, Nam Soo Kim and Jong-Hoon Kim
Chonbuk National University, South Korea
C
ancer has been viewed as a disease consisting of transformed cells, of hyper proliferative, invasive and immortal nature.
Accordingly, the anti-cancer strategies are also focused on tumor cells only. In the present study, the gastric cancer cell
(SNU-484) soluble compounds have been evaluated for its immunosuppression properties. The proteins present in the SNU-
484 soluble compounds (SC) were identified with human cytokine array. The effect of SC on rat splenocytes has been studied
with special emphasis on NK cell activity. In results, the addition of various concentration of SC did not show any significant
apoptotic or proliferation changes when compared to untreated control splenocytes. Further the incubation of splenocytes with
SC reduced the expression of NK cell markers at the transcription level. The same scenario was observed with the
in vivo
study
following 2 days of treatment. Incubation of splenocytes with SC for a longer period reduced the cytotoxic ability, further this
observation was strengthened by the reduction of CD161
+
CD3
-
(NK) cells in SC treatment. In addition tests were performed
to check whether SC can influence tumor formation in allogenic tumor model. The B16F10 melanoma cells-injected animals
developed tumor in 3 weeks, whilst the SC injected animals along B16F10 cells aggravates tumor formation, by increasing
the PI3K/AKT levels. These findings clearly demonstrate that the presence of SC can modulate immune system response that
favors the tumor formation.
Biography
Mohammad Amjad Hossain has completed his graduation from University of Development Alternative, Bangladesh and currently studying MS in Veterinary
Medicine from Chonbuk National University School of Veterinary Medicine, South Korea. He has published 2 papers in reputed journals.
mamjadh2@gmail.comMohammad Amjad Hossain et al., Toxicol Open Access 2018, Volume 4
DOI: 10.4172/2476-2067-C1-006