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Volume 4
Toxicology: Open Access
ISSN: 2476-2067
Toxicology Congress 2018
March 12-14, 2018
March 12-14, 2018 Singapore
14
th
World Congress on
Toxicology and Pharmacology
Korean red ginseng increased the immune cell activity of splenocyte
in vitro
and
in vivo
Jong-Hoon Kim, Mohammad Amjad Hossain, Adithan Aravinthan, Judith Sharmila, Hae Sook Jeong and Nam Soo Kim
Chonbuk National University, South Korea
K
orean red ginseng is a pharmacological plant that is traditionally used to improve the body’s immune functions and
ameliorate the symptoms of various diseases. However, the splenocyte activity of Korean red ginseng and its underlying
molecular and cellular mechanisms are not fully understood. In this study,
in vitro
and
in vivo
immune cell activities of Korean
red ginseng were explored. Also, Korean red ginseng was assessed for its efficacy to act as an adjuvant for the immune response of
splenocytes. The porcines were treated with different concentrations of Korean red ginseng, orally for 4 weeks. The splenocytes
isolated from Korean red ginseng-treated group showed enhanced immune cell-activities in a dose dependent manner when
compared to untreated group. Further, the intracellular levels of perforin and NKp46 were found to be significantly increased in
translational level as revealed by western blot analysis, respectively. In addition, we compared the cytotoxic activity of Korean
red ginseng-treated splenocytes against target cell such as K-562 cell for 4 weeks. The Korean red ginseng-treated splenocytes
were incubated with K-562 in a ratio of dose-dependent manner for 4 hours. Korean red ginseng-treated splenocytes showed
a significantly increased cytotoxicity in dose-dependent manner. In other hand, Korean red ginseng-untreated splenocytes
showed a less immune cell activity. Finally, Korean red ginseng exhibited
in vivo
immune activities in the animal model by
increasing the intracellular levels of perforin and NKp46 without changing the animal body weight. These results suggest that
Korean red ginseng is capable of tumor cell suppression via different molecular and cellular mechanisms, including induction
of activation of immune cells.
Biography
Jong-Hoon Kim has completed his PhD from Konkuk University, Seoul, Republic of Korea and Postdoctoral studies from Konkuk University, School of Veterinary
Medicine. He is the Director for Department of Veterinary Physiology, a premier immunotherapy research lab. He has published more than 50 papers in reputed
journals and has been serving as an Editorial Board Member of
Journal of Ginseng Research.
jhkim1@chonbuk.ac.krJong-Hoon Kim et al., Toxicol Open Access 2018, Volume 4
DOI: 10.4172/2476-2067-C1-006