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Find More Information @ March 2019 Conference Series LLC Ltd
conferenceseries LLC Ltd
19th International Conference on
Obesity, Healthcare - Nutrition & Fitness
March 18 - 19, 2019 | New York, USA
JOURNAL OF OBESITY &WEIGHT LOSS THERAPY 2019, VOLUME: 4 | DOI: 10.4172/2165-7904-C2-093 SCIENTIFIC TRACKS | DAY 1
Effects of berberine on
high fat/high sucrose
induced non alcoholic
steatohepatitis (NASH)
in experimental rats
Eman Hashem Hashem Ali Elrefaei
Tanta University, Egypt
Non-alcoholic steatohepatitis
(NASH) is the most
common chronic liver disease
in the world, characterized
by the hepatic steatosis,
inflammation, hepatocyte
injury with or without
fibrogenesis, which might lead
to cirrhosis. Berberine (BBR) is
a natural isoquinoline alkaloid
with very impressive health
benefits.
The aim of this study: To
evaluate the protective effect
of BBR in experimental NASH
induced by high fat/high-
sucrose diet in male albino
rats.
Methods: 60 male albino rats
divided randomly into four
equal groups: group I (normal
control group), group II (BBR
treated control group), group
III (NASH group) and group
IV (BBR treated NASH group).
Levels of PGC-1α in hepatic
nuclear extract were measured
by ELISA, while the activity of
cytosolic glycerol 3 phosphate
dehydrogenase (GPDH1) in
liver tissue homogenate, liver
enzymes, lipid profile and
plasma FRAP were measured
spectrophotometrically.
Results: There was a
statistically significant
decrease of hepatic PGC-
1α, plasma FRAP, serum
HDL-C along with significant
increase in the activity of
GPDH1, liver enzymes as
well as hyperlipidemia in
NASH group compared to
both normal control and BBR
treated control groups. These
pathological disturbances were
significantly ameliorated by
BBR supplementation.
Conclusion: The present study
provided unequivocal evidence
that disturbed hepatic PGC-1α
and altered redox status acted
as major contributing factors
for the pathogenesis of high-
fat/high-sucrose induced NASH
in rats. It also shed some light
on the potential therapeutic
value of BBR in NASH; partly
accredited to its hypolipidemic
and antioxidant effects, in
addition to upregulating the
levels of PGC-1α in hepatic
nuclear extracts.
Biography
Eman Elrefaei has completed
her bachelor degree in
medicine and general surgery
at age of 25years from Tanta
University, school of medicine
Egypt. Then got her MD in
medical biochemistry and
molecular biology at age of
28years.