nutrition-2016_posters-accepted-abstracts

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Volume 6, Issue 5 (Suppl) J Nutr Food Sci
ISSN:2155-9600 JNFS, an open access journal
Page 61
Notes:
Nutrition 2016
September 14-16, 2016
conferenceseries.com
September 14-16, 2016 San Antonio, USA
6th International Conference and Exhibition on
Nutrition
Li-Chun Lin, J Nutr Food Sci 2016, 6:5 (Suppl)
Isoliquiritigenin chemosensitizes to Doxorubicin and inhibits the cells growth of human uterine sarcoma cells
Li-Chun Lin
Taipei Medical University of Nutrition and Health Sciences, Taiwan
Doxorubicin (Dox) is widely used for the treatment of several cancers. However, multi-drug resistance (MDR) is a major clinical
problem and an important cause of treatment failure. How to increase chemosensitivity and reduce the dose of chemotherapeutic
agents, in order to prevent drug side effects and the development of new chemotherapeutic agents are important. Uterine sarcoma is
a rare gynecologic cancer. The patients sometimes are asymptomatic. It has the high degree of malignancy, poor prognosis and high
mortality rate. It can often be misdiagnosed as uterine fibroids. Isoliquiritigenin (ISL) is the flavonoid with chalcone structure isolated
from licorice root. ISL has been shown to possess significant anticancer activities in many cancer types. In this study, we investigated
the antitumor effects of ISL on human uterine sarcoma cancer cell MES-SA and the multi-drug resistant human uterine sarcoma
cancer cell MES-SA/Dx5 and MES-SA/DxR-1. Our present results showed that, treatment of ISL alone or combination with Dox
significantly inhibited the growth of cancer cells and increased the proportion of subG1 phase. Flow cytometry analysis indicated that
ISL induced apoptosis and necrosis. In addition, ISL enhanced the autophagy associated protein expression of LC3B and apoptosis
associated protein expression of cleaved-PARP. ISL also inhibited Bcl-2 and phospho-mTOR protein expression. Moreover, ISL
inhibited the migration of the cancer cells. Taken together, ISL can inhibit human uterine sarcoma cancer cells through apoptosis
as well as autophagy and increase the chemosensitivity to doxorubicin in multi-drug resistant human uterine sarcoma cancer cells
MES-SA/Dx5 and MES-SA/DxR-1.
Biography
Li-Chun Lin is currently a graduate student from Taipei Medical University of Nutrition and Health Sciences, Taiwan. She studies the effects of Chinese herbs and
phytochemicals on cancer disease.