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Volume 6, Issue 5 (Suppl) J Nutr Food Sci
ISSN:2155-9600 JNFS, an open access journal
Page 60
Notes:
Nutrition 2016
September 14-16, 2016
conferenceseries.com
September 14-16, 2016 San Antonio, USA
6th International Conference and Exhibition on
Nutrition
Hsin-Yuan Chen et al., J Nutr Food Sci 2016, 6:5 (Suppl)
The effect of isoliquiritigenin-induced apoptosis and autophagy of human endometrial cancer cell line
and underline mechanism
Hsin-Yuan Chen1, Tsui-Chin Huang1,2, Chi-Hao Wu1, Tzong-Ming Shieh3 and Shih-Min Hsia1
1Taipei Medical University, Taiwan
2Academia Sinica, Taiwan
3China Medical University, Taiwan
Endometrial cancer occurs in women after menopause, is the fourth most common cancer in women in the United States. In
Taiwan, due to the widespread use of hormone replacement therapy, the incidence of gynecologic cancer climbs to first place. In
recent years, western countries begin the widespread use of herbal therapies, Isoliquiritigenin (ISL), one of the active components
in licorice plant flavonoids, in past study, ISL had antioxidant, anti-inflammatory and tumor suppression effect. In this study, we
investigated the antitumor effect of ISL on human endometrial cancer in vitro and in vivo. We used human normal endometrial cell
lines, T HESCs and human endometrial cancer cell lines, Ishikawa, HEC-1A and RL95-2 as targets. To examined that the effect of
ISL on the cell proliferation, cell cycle regulation, and apoptosis or autophagy related protein expression. In addition, we conducted
in vivo experiment to confirm the inhibitory effects of ISL on cancer cell. As the current results show, ISL significantly inhibited the
viability of cancer cell in a dose dependent and time dependent manner but has little toxicity on normal cell. And also, flow cytometry
analysis indicated that ISL induced sub G1 phase arrest. Mechanistically, ISL enhanced the expression of PARP/LC3B II protein
associated with apoptosis/autophagy. Furthermore, ISL suppressed the xenograft tumor growth in vivo. These findings suggest that
ISL is a candidate agent for the treatment of human endometrial cancer and may play an important role in ISL-induced apoptosis,
autophagy and cell growth inhibition.
Biography
Hsin-Yuan Chen has completed her Master’s degree from Taipei Medical University, Taiwan. She has received First Prize in Nutrition & Biotechnology Group in
TMU 2015 Teachers and Students Joint Academic Symposium for an “Excellent Research Paper”