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Volume 7, Issue 3 (Suppl)
J Nutr Disorders Ther, an open access journal
ISSN: 2161-0509
Page 82
JOINT EVENT
&
July 27-29, 2017 Rome, Italy
Advances in Natural Medicines Nutraceuticals & Neurocognition
14
th
International Conference on Clinical Nutrition
13
th
International Congress on
The anticancer mechanisms of docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA) on
human hepatocarcinoma: a protoemic approach
Jennifer Man-Fan Wan
and
Wing-Yan Jor
The University of Hong Kong, China
O
mega-3 fatty acids have been linked to cancers prevention. However it is not clear whether there are different anticancer
mechanisms between the omega-3 fatty acids docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA). The present
study adpoted a protomic approach in order to identify specific biomarkers to define the signaling pathways that are unqiue to
both DHA and EPA. By using the non-metastatic human epatocarcinoma cell line PLC/PRF/5, we have profiled the proteins
espression of the PLC/PRF/5 cells after 72 hours treatment of DHA (200 µL) and EPA (200 µL) by the two dimensional gel
electrophoresis (2D-PAGE). Differential expressed proteins were identified by the matrix-assiated laser desorption/ionization
time of fight mass spectrometry (MALSI-TOF/TOF). Our results shows that both DHA and EPA inhibited cancer growth
and induced apoptosis. DHA posed a stronger cytotoxic effect than EPA. Differentially expressed proteins in the signalling
pathways, cell proliferation, tumor metastasis and apoptosis were found between EPA and DHA treatment. DHA suppressed
calumenin and annexin A2, which are protins affecting tumor metastatic stability and EPA down-regulated. The heterogeneous
nuclear ribonucleoprotein K (hnRNPK) and ubiquinol-cytochrome C reductase core protein 1 (UQCRC1) play a key role in the
coordination of transcriptional responses to DNA damage and in mitochondria-to-nucleus retrograde reponse, respectively.
The present study provides signature proteins associated with the anticancer mechanisms of DHA and EPA, and indicating
some functional differencs betwen the two different types of omega-3 fatty acids in the prevention of human liver cancer.
Biography
Jennifer Man-Fan Wan has completed her PhD from Southampton University and Postdoctoral studies from Harvard Medical School, Boston, USA. She is an
Assoicate Professor at the School of Biological Sciences, the University of Hong Kong. She has published more than 100 papers in reputed journals and has been
serving as an Editorial Board Member of
Nature Partner of Science of Food.
jmfwan@hku.hkJennifer Man-Fan Wan et al., J Nutr Disorders Ther 2017, 7:3(Suppl)
DOI: 10.4172/2161-0509-C1-007