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Notes:
Volume 7, Issue 3 (Suppl)
J Nutr Disorders Ther, an open access journal
ISSN: 2161-0509
Page 87
JOINT EVENT
&
July 27-29, 2017 Rome, Italy
Advances in Natural Medicines Nutraceuticals & Neurocognition
14
th
International Conference on Clinical Nutrition
13
th
International Congress on
Antigenotoxic and anti-proliferative effects of Pistacia lentiscus
Bouguellid Ghania
1
, Russo Chiara
2
, Lavorgna Margharita
2
, Piscitelli Concetta
2
, Atmani Djebbar
1
and
Isidori Marina
2
1
Université de Bejaia, Algeria
2
University of Campania “Luigi Vanvitelli”, Italy
Introduction & Aim:
Pistacia lentiscus (Anacardiaceae) is widely distributed in the Mediterranean basin ecosystem. The aerial
parts are used in folk medicine as stimulants, diuretics, and to treat hypertension, eczema, stomach aches and jaundice. Fruits
are consumed raw or roasted; while their oil is used internally in the treatment of ulcers or externally to heal psoriasis. Previous
studies led to the quantification and/or identification of many constituents of different chemical classes such as flavonoids,
anthocyanins, phenolic acids (gallic acid, digallic acid, catechin), triterpenoids, and tannins. These constituents are responsible
for antioxidant and several pharmacological properties, including hepatoprotective, anti-atherogenic, anti-ulcer and anti-
inflammatory. In this study, our aim was to examine the antigenotoxic and antiproliferative potential of different leaves and
fruit extracts of
P. lentiscus
.
Materials & Methods:
The genotoxic/antigenotoxic activities of
P. lentiscus
were evaluated using the SOS repair response
induction assay on
Salmonella typhimurium
TA 1535/pSK 1002 in the presence and absence of metabolic activation system.
The anti-proliferative effect was investigated by MTT assay for cytotoxicity on human hepatoma cancer cell line (Hepg-2).
Results:
In preliminary studies, both leaves and fruit extracts did not show genotoxic activity when tested with
S. typhimurium
TA 1535 psK/1002 strains at concentration up to 1000 µg/ml in the absence and presence of S9 metabolic activation. However,
a slight genotoxic effect was observed with the crude ethanolic extract of leaves at 1000 µg/ml; besides, when tested with
500 and 100 µg/ml, none of this effect was observed. Otherwise, when combined with mutagens 4-nitroquinoline and 2-
aminoanthracene, different extracts showed moderate protective effect and a strong protective effect with crude ethanolic
extract and the aqueous fraction of ethyl acetate. Treatment of the human liver cancer cell line Hep G2 with different extracts
of leaves and fruit of
P. lentiscus
, at a concentration range of 25–2500 µg/ml, exhibited a fairly good anti proliferative effects, the
most active effect was attributed to the ethanolic and aqueous fraction obtained from ethyl acetate of leaves with IC50 value of
271.5 and 249 µg/ml respectively.
Conclusion:
These results provide experimental support for the therapeutic virtues of Pistacia lentiscus extracts.
Biography
Bouguellid Ghania is pursuing her PhD and her thesis concentrates on the evaluation of the genotoxic/antigenotoxic activity of medicinal plant extracts, in order to
remove the doubt about the toxicity of the extracts before envisaging their uses in therapy.
bouguellid_ghania@live.frBouguellid Ghania et al., J Nutr Disorders Ther 2017, 7:3(Suppl)
DOI: 10.4172/2161-0509-C1-007