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Volume 3
Diagnostic Pathology: Open Access
ISSN: 2476-2024
Laboratory Medicine 2018
June 25-26, 2018
June 25-26, 2018 | Berlin, Germany
13
th
International Conference on
Laboratory Medicine & Pathology
Combining 2D angiogenesis and 3D osteosarcoma microtissues to improve vascularization
Hassan Chaddad
INSERM, UMR 1260, Regenerative Nanomedicine, FMTS, University of Strasbourg, France
Introduction:
The number of patients suffering from cancers worldwide is increasing, and one of the most challenging issues
in oncology continues to be the problem of developing active drugs economically and in a timely manner. Considering the high
cost and time-consuming nature of the clinical development of oncology drugs, better pre-clinical platforms for drug screening
are urgently required. So, there is need for high-throughput drug screening platforms to mimic the
in vivo
microenvironment.
Angiogenesis is now well known for being involved in tumour progression, aggressiveness, emergence of metastases, and also
resistance to cancer therapies.
Materials & Methodology:
In this study, to better mimic tumour angiogenesis encountered
in vivo
, we used 3D culture of
osteosarcoma cells (MG-63) that we deposited on 2D endothelial cells (HUVEC- Human Umbilical vein Endothelial Cells)
grown in monolayer. Combination 2D HUVEC/3D MG-63 was characterized by Indirect immunofluorescence, Scanning
electron microscopy, Optical microscopy and mRNA expression (qPCR).
Results:
We reported that endothelial cells combined with tumor cells were able to form a well-organized network, and those
tubule-like structures corresponding to new vessels infiltrate tumor spheroids. These vessels presented a lumen and expressed
specific markers as CD31 and collagen IV. The combination of 2D endothelial cells and 3D microtissues of tumor cells also
increased expression of angiogenic factors as VEGF, CXCR4 and ICAM1.
Conclusions:
The cell environment is the key point to develop tumor vascularization
in vitro
and to be closer to tumor
encountered
in vivo
.
Hassan.chaddad@unistra.frDiagn Pathol Open 2018, Volume 3
DOI: 10.4172/2476-2024-C1-003