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conferenceseries
.com
Volume 3
Diagnostic Pathology: Open Access
ISSN: 2476-2024
Laboratory Medicine 2018
June 25-26, 2018
June 25-26, 2018 | Berlin, Germany
13
th
International Conference on
Laboratory Medicine & Pathology
ANTIGLYCATIONEFFECTOFWHITTONROOT(EULOPHIANUDA) IN-VITROCONDITION
IN RELATION TO DIABETES MELLITUS
Dinesh Prasad Yadav
and
Sonia Sharma
B & C Medical College Teaching Hospital and Research Center Pvt Ltd, Nepal
N
on enzymatic glycation takes place when elevated levels of reduced sugars react with amino groups of proteins and is
called as advanced glycation end products (AGEs) responsible for diabetes mellitus. Hydroalcoholic extract of whitton
root (
Eulophia nuda
) was tested for
in-vitro
inhibition of non-enzymatic glycation of IgG (immunoglobulin G). Plant extracts
have their own importance and now being studied extensively due to little or no side effects in all aspects of life sciences
from botany to medicine in biochemical research. In present study whitton root was selected and used to check the glycation
inhibitory activity
in vitro
condition. Various combinations of glucose, protein and whitton root extracts were made under
in
vitro
conditions and their activity was monitored with trichloroacetic acid treatment method at 350 nm. Glycated products/
AGEs was more with high glucose and high protein concentration and these were decreased by highest concentration of
whitton root extract i.e. 30 mg/mL or 300 μL. Lower concentrations of plant extract produced either no or least response
against advanced glycation end products (AGEs).
ddineshh2004@gmail.comDiagn Pathol Open 2018, Volume 3
DOI: 10.4172/2476-2024-C1-003