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.com
Volume 5, Issue 3 (Suppl)
J Infect Dis Ther, an open access journal
ISSN:2332-0877
Infectious Diseases 2017
August 21-23, 2017
3
rd
Annual Congress on
Infectious Diseases
August 21-23, 2017 San Francisco, USA
Antimicrobial mechanism of n-hexane and dichloromethane extract of
Cledodendron phlomidis
on
Bacillus licheniformis
Alok Kumar Yadav, Manisha Rani, Manjoo Rani
and
Nand K Singh
Motilal Nehru National Institute of Technology Allahabad, India
R
esistance of microorganism against antibiotics is a serious concern in both developed and developing country. The
resistance of these microorganisms against these microorganism force researches to search new antimicrobial compound
from plant source to inhibit their pathogenicity. Though plants phytochemicals have antimicrobial activity but the mechanism
behind their action is limited. Cledodendron philomidis n-hexane and dichloromethane extract were isolated by soxhlet to
assess the antimicrobial potential by agar well diffusion over
Bacillus licheniformis
, a common food poisoning bacterium.
The mechanism of action behind their antimicrobial activity was determined using DNA fragmentation, scanning electron
microscopy, fluorescent activation of cell sorting (FACS). The well diffusion assay showed an inhibition zone of 19 and 15.5 mm
in n-hexane and dichloromethane hexane extract of Cledodendron philomidis. The DNA fragmentation showed significance of
apoptosis after an incubation of bacteria with n-hexane and dichloromethane extract of
Cledodendron phlomidis
for 72 hours.
Scanning electron microscope revealed adherence of plant extract of n-hexane and dichloromethane extract of
Cledodendron
phlomidis
and degradation of bacterial membrane. The flow cytometer revealed that incubating Bacillus subtilis with extract
resulted in decrease in fluorescence intensity that gives significant evidence for reduction of membrane potential and hence
suggesting the mechanism of antibacterial activity involves disruption of membrane potential. The antimicrobial potential of
these extracts can be utilized for the treatment of various food born infections in spite of industrially produced antibiotics.
Biography
Alok Kumar Yadav is pursuing his PhD in the Department of Biotechnology, Motilal Nehru National Institute of Technology, and Allahabad under the supervision of
Dr. N K Singh working on the application herbal and dietary phytochemicals for human health care. He has published 3 abstracts in international conferences and
submitted one research article in reputed journal.
alok25jul1989@gmail.comAlok Kumar Yadav et al., J Infect Dis Ther 2017, 5:3 (Suppl)
DOI: 10.4172/2332-0877-C1-027