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conferenceseries.com
Volume 5, Issue 3 (Suppl) J Infect Dis Ther, an open access journal
ISSN:2332-0877 Infectious Diseases 2017
August 21-23, 2017
3rd Annual Congress on
Infectious DiseasesAugust 21-23, 2017 San Francisco, USA
The role of B-1 cells in reducing susceptibility to oral infection by Encephalitozoon cuniculi
Adriano Pereira, Denise Langanke Dos Santos, Anuska Marcelino Alvares-Saraiva, José Guilherme Xavier, Paulo Ricardo Dell’Armelina Rocha, Elizabeth Christina
Perez Hurtado and Maria Anete Lallo
São Paulo State University, Brazil
Microsporidia are intracellular pathogens that cause severe disease in immunocompromised humans and animals. We
recently demonstrated that XID mice are more susceptible to Encephalitozoon cuniculi infection by peritoneal route,
evidencing the role of B-1 cells in the resistance against infection. The present study aimed to evaluate the mechanisms of
resistance and susceptibility against E. cuniculi oral infection, including the role of B-1 cells, using BALB/c and BALB/c XID
mice. We used flow cytometry to characterize the immune cells in the peritoneal cavity, spleen and Payer’s patches and also to
quantify the serum levels of Th1, Th2, Th17 profile cytokines. Moreover, histopathology was performed in the intestines, lungs
and liver. No clinical symptoms were observed in infected animals but histopathological analysis revealed lymphoplasmocytic
enteritis with degeneration of the apexes of the villi in all infected groups. Higher parasite burden was observed in infected
BALB/c XID mice. In the spleen, all infected mice showed a decrease of B-2, T CD4+ and T CD8+ cells. B-1 and B-2 cells
decreased in the peritoneal cavity of infected BALB/c XID and XID+B-1 mice. Macrophages increased only in infected BALB/c
mice. Pro-inflammatory cytokines increased mostly in infected XID+B-1 mice. Together, the present results demonstrated
that BALB/c XID mice were more susceptible to encephalitozoonosis and, also, the B-1 cells role in the control of the immune
response against E. cuniculi oral infection.
Biography
Adriano Pereira is a Teacher in the areas of health and biological sciences at São Camilo University, São Paulo, Brazil. He has done Master’s degree in Veterinary
Medicine and PhD in Environmental and Experimental Pathology. His research involves studying microsporidia with a focus on biology and immune response
against this emerging and opportunistic pathogen.
Adriano Pereira et al., J Infect Dis Ther 2017, 5:3 (Suppl)
DOI: 10.4172/2332-0877-C1-027