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conferenceseries
.com
Volume 5, Issue 3 (Suppl)
J Infect Dis Ther, an open access journal
ISSN:2332-0877
Infectious Diseases 2017
August 21-23, 2017
3
rd
Annual Congress on
Infectious Diseases
August 21-23, 2017 San Francisco, USA
Evaluation of the enzyme iron superoxide dismutase (Fe-SODe) as a molecular marker in the
diagnosis and identification of
Leishmania spp
in the school-age population of central Mexico
Catalina A López-González
1
, Manuel Sánchez-Moreno
2
, Juan Mosqueda-Gualito
1
, Hernández-Montiel Hebert Luis
1
, José A de Diego-Cabrera
3
, María Elena Villagrán-
Herrera
1
, Ricardo F Mercado-Curiel
1
and
Nicolás Camacho-Calderón
1
1
UAQ, Mexico
2
UGR, Spain
3
UAM, Spain
Statement of the Problem:
The current method for diagnosing a
leishmanias
is infection in Mexico is to perform a biopsy of
the effected tissue, by observing amastigotes within macrophages. This procedure is very invasive for the patient (1). Another
method uses indirect immunofluorescence. Since it uses the entire parasite, this implies low sensitivity and specificity (2).
In addition, the limitation becomes evident when the parasitaemia in clinical samples is low (3). These methods do not
discriminate between species of
Leishmania
, impeding optimal treatment for the patient (4, 5). The purpose of this study was
to evaluate the potential of the enzyme iron superoxide dismutase (Fe-SODe) excreted by
Leishmania spp
, used as an antigenic
fraction by ELISA and western blot as a reference test for the search anti-
Leishmania
antibodies in central Mexico’s school-age
population.
Methodology:
131 serums from 6-12 year old children were collected and analyzed by the ELISA and western blot, using the
homogenate (H) and Fe-SODe like antigen.
Findings:
A prevalence of 22.1% (29/131) for L. mexicana, 9.9% (13/131) for L. infantum and 14.5% (19/131) for L Braziliensis.
WB-FeSODe reported 23.66% (31/131) for L. mexicana, 9.9% (13/131) for L. infantum and 12.97% (17/131) for L. braziliensis.
The sensitivity obtained for ELISA-Fe-SODe on WB was 82.4% for L. braziliensis, 92.3% for L. infantum and 93.5% for L.
mexicana. The specificity obtained with this technique was 95.6% for L. braziliensis, 100% for L. mexicana and 99.2% for L.
infantum. The Kappa value was 0.743 for L. braziliensis, 0.957 for L. mexicana and 0.915 for L. infantum.
Conclusion & Significance:
A prevalence of 22.1% was reported for L. mexicana, 9.9% for L. braziliensis and 14.5% for L.
infantum in urban areas of Mexico. In addition, this study highlights the importance of the use of the enzyme Fe-SODe as a
useful diagnostic tool, practical and economical for the diagnosis
Leishmania spp
.
Biography
Catalina A López-González is pursuing her Master’s in Biomedicine. She has experience working with the diagnosis and treatment of vector-borne diseases. This
new method mentioned above of diagnosis has been evaluated in conjunction with the University of Granada (Spain), to develop a test that has high precision and
specificity in the detection of
Leishmania spp
.
catalina-74114@hotmail.comCatalina A López-González et al., J Infect Dis Ther 2017, 5:3 (Suppl)
DOI: 10.4172/2332-0877-C1-027