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conferenceseries
.com
Volume 5, Issue 3 (Suppl)
J Infect Dis Ther, an open access journal
ISSN:2332-0877
Infectious Diseases 2017
August 21-23, 2017
3
rd
Annual Congress on
Infectious Diseases
August 21-23, 2017 San Francisco, USA
Immunohistochemistry approach in encephalitozoonosis
Adriano Pereira
and
Maria Anete Lallo
São Paulo State University, Brazil
E
ncephalitozoonosis is a common disease of a wide range of mammalian hosts caused by
Encephalitozoon cuniculi
and the
detection of this pathogen in tissue samples is considered difficult. Microsporidia spores of
E. cuniculi
can be observed
in histological sections stained with routine dyes in tissues of experimentally infected animals in the laboratory because the
amount of spores is generally large, but in veterinary clinics of domestic and wild animals these stains are often not sufficient
for diagnosis. Then, for these cases a variety of techniques, including special staining methods, immunohistochemistry (IHC),
electron microscopy and molecular methods are used for diagnosis and exclusion of other microorganisms. The aim of this
study was to describe about the use of IHC for the detection of
E. cuniculi
in tissue samples that have been published to
some groups of researchers and veterinary pathologists throughout the world. An English literature search was done through
databases (MEDLINE; NCBI, Bethesda, MD, USA) in order to examine publications. We considered papers from 1993 to
today that described IHC analysis performed using formalin fixed and paraffin-embedded tissue sections. The diagnosis of
encephalitozoonosis using IHC has been made in rabbits (brain, kidneys, lungs, heart, liver, eyes, and spleen), dogs (eyes),
horse (villi ofvallantochorion), South American fur seal (lungs, spleen and kidneys), squirrel monkey (brain), emperor
tamarins (blood capillaries, arteries, heart, liver, lung, brain and Kidney), cats (brain and kidney), cotton-top tamarins (kidneys
and blood), chicken (esophagus, intestine, liver, kidneys, heart, skeletal muscle and brain) and snow leopard (eyes).
E. cuniculi
was successfully identified in different kind of tissues using IHC. Based on our results, we suggest that IHC should be regarded
as a useful tool both for specific demonstration of
E. cuniculi
and for its localization within tissues helping researchers and
veterinary pathologists for the diagnosis of encephalitozoonosis.
Biography
Adriano Pereira is a Teacher in the areas of health and biological sciences at São Camilo University, São Paulo, Brazil. He has done Master’s degree in Veterinary
Medicine and PhD in Environmental and Experimental Pathology. His research involves studying microsporidia with a focus on biology and immune response
against this emerging and opportunistic pathogen.
biomedadriano@yahoo.com.brAdriano Pereira et al., J Infect Dis Ther 2017, 5:3 (Suppl)
DOI: 10.4172/2332-0877-C1-027