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Volume 8, Issue 3 (Suppl)
J Clin Cell Immunol, an open access journal
ISSN: 2155-9899
Euro Immunology 2017
June 29-July 01, 2017
June 29-July 01, 2017 Madrid, Spain
8
th
European
Immunology Conference
Regulation of TIM-3 expression in T cells by tumor-conditioned media
Sun Park
and
Su-jin Yun
Ajou University, South Korea
T
cell immunoglobulin- and mucin-domain-containing molecule-3 (TIM-3) is well known as one of the immune check point
molecules. TIM-3 expression is increased on exhausted T cells and senescent T cells in numerous immune diseases including
cancers. However, the regulatory mechanisms of TIM-3 expression in cancers have not been well studied. Using Jurkat T cells, we
examined TIM-3 regulatory mechanisms in condition similar to tumor microenvironment. TIM-3 mRNA and protein levels were
increased by co-culture of Jurkat T cells with tumor cell lines and by incubation of them in tumor cell conditioned media. Given that
cyclic adenosine monophosphate (cAMP) can be transferred from tumor cells to T cells, we examined the effect of cAMP signaling on
TIM-3 expression. It was promoted by intracellular elevation of cAMP concentration and activation of cAMP downstream pathways.
Further, inhibition of cAMP downstream pathway attenuated TIM-3 expression in Jurkat T cells cultured in tumor-CM as well as in
Jurkat T cells stimulated with a cAMP elevating agent. Conclusively, this study suggests that TIM-3 expression in Jurkat T cells may
be induced by tumor CM through activation of cAMP pathway.
Biography
Immune regulation has important roles in various immune diseases. The authors have studied the regulatory mechanisms and function of TIM-3 in various cells
and in an
in vivo
tumor model. The authors revealed the involvement of MEK and c-jun in TIM-3 expression by CD4+ T cells. Additionally, they reported that the
efficacy of tumor vaccine can be up-regulated by TIM-3 pathway blockade and the IL-2 production is decreased in CD4+ T cells expressing TIM-3 through NFAT
dephosphorylation and AP-1 transcription.
sinsun@ajou.ac.krSun Park et al., J Clin Cell Immunol 2017, 8:3(Suppl)
DOI: 10.4172/2155-9899-C1-037