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Volume 8, Issue 3 (Suppl) J Clin Cell Immunol, an open access journal
ISSN: 2155-9899 Euro Immunology 2017
June 29-July 01, 2017
June 29-July 01, 2017 Madrid, Spain
8th European
Immunology Conference
Serum protein pattern associated with severe manifestations of SLE: Organ damage and lupus nephritis
Kriegova E, Petrackova A, Smrzova A, Schubertova M, Schneiderova P, Dyskova T, Skacelova M, Mrazek F and Horak P
Palacky University Olomouc, Czech Republic
Statement of the Problem: Systemic lupus erythematosus (SLE) is a remarkably heterogeneous autoimmune disease. The greatest
challenges remain in management of organ damage and lupus nephritis (LN), one of the most feared phenotypes in SLE. Despite
tremendous effort, our knowledge on serum protein pattern in this severe SLE manifestation is still limited. The purpose of this study
was to investigate the serum protein pattern of organ damage and LN in SLE using a highly sensitive multiplex Proximity Extension
Immunoassay (PEA) on 92 inflammation-related proteins.
Methodology & Theoretical Orientation: We enrolled 75 Czech patients with SLE, subgroups were formed according to the organ
damage (assessed by the SLICC/ACR damage index, SDI) (SDI≥1, n=42; SDI=0, n=33) and biopsy-proven presence of LN (no LN,
n=48; LN, n=27). The serum levels of 92 inflammation-related proteins were assessed by PEA (Proseek Multiplex, Olink Bioscience,
Sweden). Statistical tests (Student t-test, Benjamini-Hochberg correction) were performed using GenEx (Sweden), p-value≤0.05 was
considered as significant.
Findings: SLE patients with organ damage had elevated serum levels of IL-8, CCL2, IL-6, CCL11, FGF21, MMP10, IL-18, CCL3, FGF5
and FGF23 comparing to those without organ damage. Of these, enhanced levels of CCL11, MMP10s and CCL2 were informative for
identification of patients with organ damage. Importantly, IL-8 and MMP10 also correlated with disease activity (r≤0.355, p≤0.002).
Comparing patients with/without LN, elevated levels of CSF1, sCX3CL and GDNF, sCD40 (Pcorr<0.05) were detected and showed
usefulness in prediction of this severe SLE manifestation. Although all upregulated proteins correlated with disease activity, the best
correlation was observed for sCX3CL1 and GDNF (r≥0.403, p≤0.0003).
Conclusion & Significance: This highly sensitive PEA analysis identified serum pattern of organ damage and LN, with many novel
candidate proteins detected. Their exact role and suitability as biomarkers in SLE deserves further investigation.
Biography
Dr. Eva Kriegova is an immunologist at the Faculty of Medicine and Dentistry, Palacky University Olomouc, Czech Republic. She is leader of Molecular Immunology
group and her research interest is focused on molecular mechanisms of immune processes ongoing in inflammatory lung disorders and autoimmune diseases,
hematological malignancies and orthopaedic disorders.
Anna Petrackova is a PhD student of Immunology at Palacky University Olomouc, Czech Republic. Her research is focused on gene expression studies of
inflammatory mediators in autoimmune disorders and hematological malignancies.
Kriegova E et al., J Clin Cell Immunol 2017, 8:3(Suppl)
DOI: 10.4172/2155-9899-C1-037