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Volume 7, Issue 3(Suppl)
J Biotechnol Biomater, an open access journal
ISSN: 2155-952X
Euro Biotechnology 2017
September 25-27, 2017
17
th
EURO BIOTECHNOLOGY CONGRESS
September 25-27, 2017 Berlin, Germany
Orthogonal regulation of gene expression in yeast using plant-derived transcription factors
Gita Naseri
University of Potsdam, Germany
C
entral goal for synthetic biology is the regulated expression of genes for establishing complex protein expression systems. Artificial
transcription factors are one possibility for regulation of gene expression in an orthogonal control system. Presented data show
the use of plant-derived artificial transcription factors to establish orthogonal regulators in yeast. The library consists of more than
100 members build from different DNA-binding sites, activation domains and corresponding synthetic promoters. Functionality of
the library members is shown by GFP expression and its flow cytometric quantitation.
Biography
Gita Naseri completed her Master’s degree in Plant Biotechnology from University of Guilan (Iran) followed by several years as Technical Manager in laboratories
of the Rice Research Center (Rasht, Iran) and the laboratory of Plant Protection Clinic (Fouman, Iran). In 2013, she joined the group for synthetic biosystems at
Potsdam University (Germany) as a PhD student. She has published first data of her thesis in
ACS Syn Biol
in 2017.
naseri@uni-potsdam.deGita Naseri, J Biotechnol Biomater 2017, 7:3(Suppl)
DOI: 10.4172/2155-952X-C1-076